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Reduced Osteogenic Differentiation Potential In Vivo in Acute Myeloid Leukaemia Patients Correlates with Decreased BMP4 Expression in Mesenchymal Stromal Cells

Azevedo PL, Dias RB, Nogueira LP, Maradei S, Bigni R, Aragao JSR, Abdelhay E, Binato R

The osteogenic differentiation potential of mesenchymal stromal cells (hMSCs) is an essential process for the haematopoiesis and the maintenance of haematopoietic stem cells (HSCs). Therefore, the aim of this work...
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The Role of miR-34c-5p in Osteogenic Differentiation of Bone Marrow Mesenchymal Stem Cells

Liu B, Gan W, Jin Z, Wang M, Cui G, Zhang H, Wang H

Background and Objectives: Osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) plays a critical role in the success of lumbar spinal fusion with autogenous bone graft. This study aims...
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Effect of TNF-α and IL-6 on Compact Bone-Derived Cells

Zhang Y, Li X, Chihara T, Dong H, Kagami H

Background: Although bone tissue engineering has already been applied clinically, its regeneration efficacy is not always sufficient. Local inflammatory cytokines are considered as the major factors that induce apoptosis of transplanted...
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Frozen-thawed gelatin-induced osteogenic cell sheets of canine adipose-derived mesenchymal stromal cells improved fracture healing in canine model

Yoon Y, Jung T, Afan Shahid M, Khan IU, Kim WH, Kweon OK

We assessed the efficacy of frozen-thawed gelatin-induced osteogenic cell sheet (FT-GCS) compared to that of fresh gelatin-induced osteogenic cell sheet (F-GCS) with adipose-derived mesenchymal stromal cells (Ad-MSCs) used as the...
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Bone Morphogenetic Protein 2-Conjugated Silica Particles Enhanced Early Osteogenic Differentiation of Adipose Stem Cells on the Polycaprolactone Scaffold

Kim KJ, Choi MS, Shim JH, Rhie JW

BACKGROUND: Silica particles (SPs) induce cell proliferation and osteogenic differentiation. We reported that SPs in the scaffold induced early stage osteogenic differentiation. METHODS: A polycaprolactone (PCL) scaffold was fabricated with a...
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Conditioned Medium Enhances Osteogenic Differentiation of Induced Pluripotent Stem Cell-Derived Mesenchymal Stem Cells

Zhong S, He X, Li Y, Lou X

BACKGROUND: Recent studies have shown that induced pluripotent stem cells (iPSCs) could be differentiated into mesenchymal stem cells (MSCs) with notable advantages over iPSCs per se. In order to promote...
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Determining Osteogenic Differentiation Efficacy of Pluripotent Stem Cells by Telomerase Activity

Zhang S, Sun Y, Sui Y, Li Y, Luo Z, Xiao X, Zhou P, Wei S

BACKGROUND: Bone tissue engineering based on pluripotent stem cells (PSCs) is a new approach to deal with bone defects. Protocols have been developed to generate osteoblasts from PSCs. However, the...
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In Vitro and In Vivo Osteogenesis of Human Orbicularis Oculi Muscle-Derived Stem Cells

Liu G, Liao C, Chen X, Wu Y

BACKGROUND: Cell-based therapies for treating bone defects require a source of stem cells with osteogenic potential. There is evidence from pathologic ossification within muscles that human skeletal muscles contain osteogenic...
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Effects of RetroMTA on osteoblastic differentiation in MC3T3-E1 cells

Lee HI, Choi SH, Jang JH, Chang HS, Hwang YC, Hwang IN, Lee BN, Oh WM

Mineral trioxide aggregate (MTA) provides a very good sealing, acceptable biocompatibility, dentin bridge formation and osteo-inductive effect. However, MTA has a few drawbacks. RetroMTA including calcium-zirconia complex has been developed...
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Different Bone Healing Effects of Undifferentiated and Osteogenic Differentiated Mesenchymal Stromal Cell Sheets in Canine Radial Fracture Model

Yoon Y, Khan IU, Choi KU, Jung T, Jo K, Lee SH, Kim WH, Kim DY, Kweon OK

Cell sheets technology is being available for fracture healing. This study was performed to clarify bone healing mechanism of undifferentiated (UCS) and osteogenic (OCS) differentiated mesenchymal stromal cell (MSC) sheets...
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In Vitro Osteogenic Differentiation of Human Mesenchymal Stem Cells from Jawbone Compared with Dental Tissue

Pettersson LF, Kingham PJ, Wiberg M, Kelk P

Autologous bone transplantation is the current gold standard for reconstruction of jawbone defects. Bone regeneration usingmesenchymal stem cells (MSC) is an interesting alternative to improve the current techniques,which necessitate a...
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Icaritin, a Flavonoid Derived from the Herb Epimedium, Promotes Osteogenic Differentiation of MC3T3-E1 Cells

Park DB, Lee HS, Ko SH

Osteoporosis is a metabolic bone disease that is characterized by low bone mass resulting from an increase in bone resorption relative to bone formation. The most current therapies for osteoporosis...
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Enhancement of Osteogenic Differentiation by Combination Treatment with 5-azacytidine and Thyroid-Stimulating Hormone in Human Osteoblast Cells

Sun HJ, Song YS, Cho SW, Park YJ

BACKGROUND AND OBJECTIVES: The role of thyroid-stimulating hormone (TSH) signaling on osteoblastic differentiation is still undetermined. The aim of this study was to investigate the effects of 5-aza-2′-deoxycytidine (5-azacytidine) on...
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Coculture Effects on the Osteogenic Differentiation of Human Mesenchymal Stromal Cells

Hayrapetyan A, Surjandi S, Lemsom EE, Wolters MM, Jansen JA, van den Beucken JJ

Cell-based bone regeneration is generally pursued based on single cell type approaches, for which human adipose tissue-derived mesenchymal stromal cells (AT-MSCs) are frequently used, owing to their easy accessibility and...
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Indirect Co-Culture of Stem Cells from Human Exfoliated Deciduous Teeth and Oral Cells in a Microfluidic Platform

Kang KJ, Ju SM, Jang YJ, Kim J

Oral epithelial-mesenchymal interactions play a key role in tooth development and assist differentiation of dental pulp. Many epithelial and mesenchymal factors in the microenvironment influence dental pulp stem cells to...
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Mechanisms of Vascular Calcification: The Pivotal Role of Pyruvate Dehydrogenase Kinase 4

Leem J, Lee IK

Vascular calcification, abnormal mineralization of the vessel wall, is frequently associated with aging, atherosclerosis, diabetes mellitus, and chronic kidney disease. Vascular calcification is a key risk factor for many adverse...
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Mesengenic Differentiation: Comparison of Human and Rat Bone Marrow Mesenchymal Stem Cells

Scuteri A, Donzelli E, Foudah D, Caldara C, Redondo J, D'Amico G, Tredici G, Miloso M

BACKGROUND AND OBJECTIVES: Cellular therapies using Mesenchymal Stem Cells (MSCs) represent a promising approach for the treatment of degenerative diseases, in particular for mesengenic tissue regeneration. However, before the approval...
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Translational Research: Palatal-derived Ecto-mesenchymal Stem Cells from Human Palate: A New Hope for Alveolar Bone and Cranio-Facial Bone Reconstruction

Grimm WD, Dannan A, Giesenhagen B, Schau I, Varga G, Vukovic MA, Sirak SV

  • KMID: 2054556
  • Int J Stem Cells.
  • 2014 May;7(1):23-29.
The management of facial defects has rapidly changed in the last decade. Functional and esthetic requirements have steadily increased along with the refinements of surgery. In the case of advanced...
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Effects of nanoscale ridge/groovepattern arrayed surface on in vitro differentiation of multi-potent pulp cells derived from human supernumerary teeth

Kim D, Jo H, Lee J, Kim K, Roh S

  • KMID: 1709610
  • Int J Oral Biol.
  • 2013 Dec;38(4):161-167.
Human dental pulp stem cells (DPSCs) are multi-potent mesenchymal stem cells that have several differentiation potentials. An understanding of the tissues that differentiate from these cells can provide insights for...
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Effects of CoCl2 on Osteogenic Differentiation of Human Mesenchymal Stem Cells

Moon YH, Son JW, Moon JS, Kang JH, Kim SH, Kim MS

  • KMID: 2170781
  • Int J Oral Biol.
  • 2013 Sep;38(3):111-119.
OBJECTIVE: To investigate the effects of the hypoxia inducible factor-1 (HIF-1) activation-mimicking agent cobalt chloride (CoCl2) on the osteogenic differentiation of human mesenchymal stem cells (hMSCs) and elucidate the underlying...
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