Exp Mol Med.
2012 Aug;44(8):465-472.
B cell-associated immune profiles in patients with end-stage renal disease (ESRD)
- Affiliations
-
- 1Conversant Research Consortium in Immunologic Disease, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul 137-040, Korea. yangch@catholic.ac.kr, sjnam@skku.edu
- 2Transplant Research Center, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul 137-040, Korea.
- 3Division of Nephrology, Department of Internal Medicine, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul 137-040, Korea.
- 4Rheumatism Research Center, Catholic Institute of Medical Science, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul 137-040, Korea.
- 5Department of Hematology, Catholic Blood and Marrow Transplantation Center, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul 137-040, Korea.
Abstract
- Most of the previous studies on immune dysregulation in end-stage renal disease (ESRD) have focused on T cell immunity. We investigated B cell subpopulations in ESRD patients and the effect of hemodialysis (HD) on B cell-associated immune profiles in these patients. Forty-four ESRD [maintenance HD patients (n = 27) and pre-dialysis patients (n = 17)] and 27 healthy volunteers were included in this study. We determined the percentage of B cell subtypes, such as mature and immature B cells, memory B cells, and interleukin (IL)-10+ cells, as well as B cell-producing cytokines (IL-10, IL-4 and IL-21) by florescent activated cell sorting (FACS). B cell-associated gene expression was examined using real-time PCR and B cell producing cytokines (IL-10, IL-4 and IL-21) were determined using an enzyme-linked immunosorbent assay (ELISA). The percentage of total B cells and mature B cells did not differ significantly among the three groups. The percentages of memory B cells were significantly higher in the pre-dialysis group than in the HD group (P < 0.01), but the percentage of immature B cells was significantly lower in the pre-dialysis group than in the other groups. The percentages of IL-10-expressing cells that were CD19+ or immature B cells did not differ significantly (P > 0.05) between the two subgroups within the ESRD group, but the serum IL-10 concentration was significantly lower in the pre-dialysis group (P < 0.01). The results of this study demonstrate significantly altered B cell-associated immunity. Specifically, an imbalance of immature and memory B cells in ESRD patients was observed, with this finding predominating in pre-dialysis patients.
MeSH Terms
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Adaptor Proteins, Signal Transducing/genetics
Adult
Antigens, CD19/metabolism
B-Lymphocyte Subsets/immunology/metabolism
B-Lymphocytes/*immunology/metabolism
Cytokines/biosynthesis
Female
Humans
Immunophenotyping
Interleukin-10/metabolism
Kidney Failure, Chronic/*immunology/metabolism
Leukocytes, Mononuclear/metabolism
Male
Middle Aged
Proto-Oncogene Proteins/genetics
T-Lymphocytes, Regulatory/immunology/metabolism
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