Exp Mol Med.  2008 Feb;40(1):98-108. 10.3858/emm.2008.40.1.98.

Alterations of proliferative and differentiation potentials of human embryonic stem cells during long-term culture

• Park YB
• Kim YY
• Oh SK
• Chung SG
• Ku SY
• Kim SH
• Choi YM
• Moon SY

• KMID: 1103593
• DOI: http://doi.org/10.3858/emm.2008.40.1.98

Abstract

Human embryonic stem cells (hESCs) are considered to be able to stably maintain their characteristics in vitro for prolonged periods, but we had previously encountered changes in proliferative ability and differentiation potential during extended culture of hESCs. Therefore, we investigated the proliferative ability and differentiation potential of hESCs during long-term culture. The hESCs, SNUhES3, were used to analyze population-doubling time, proliferation rate and differentiation potential. We classified hESCs into three groups according to culture period. Ten colonies of hESCs for each group were daily measured colony area and population-doubling time was assessed by the changes of colony area. Proliferation rate of hESCs was measured by 5-bromo-2'-deoxyuridine (BrdU) assay and telomerase activity. To evaluate differentiation potentials for hESCs, expression levels of undifferentiated and/or differentiated hESCs markers were examined by FACS, RT-PCR and immunostaining. Population-doubling time of early passage hESCs was longer than those of middle or late passage. Proliferative ability of hESCs was accelerated depending on culture periods. Cellular morphologies and the expression level of each three germ layer markers were obviously different from each passage of reattached embryoid bodies (EBs) after spontaneous differentiation. Differentiated cells of late passage expressed higher levels of undifferentiated markers such as Oct4 and SSEA4 than those of early and middle passage. But differentiated cells of early and middle passage expressed higher level of differentiated state markers, Nestin (ectoderm), Brachyury (mesoderm), HNF3beta (endoderm). From these results, it can be inferred that hESCs show higher proliferative abilities and reduced differentiation potentials as the passage number increased. Therefore, we conclude that early passage hESCs could be more suitable than middle and late passage hESCs in differentiation studies.

Keyword

cell differentiation; cell proliferation; embryonic stem cells; humans

MeSH Terms

Biological Markers/metabolism
Bromodeoxyuridine/metabolism
*Cell Differentiation
Cell Proliferation
Cells, Cultured
Cyclins/metabolism
DNA-Binding Proteins/genetics/metabolism
Embryonic Stem Cells/*cytology/enzymology
Flow Cytometry
Gene Expression Regulation
Homeodomain Proteins/genetics/metabolism
Humans
Karyotyping
Octamer Transcription Factor-3/genetics/metabolism
Telomerase/metabolism
Time Factors