Exp Mol Med.  2007 Apr;39(2):222-229.

Resveratrol stimulates glucose transport in C2C12 myotubes by activating AMP-activated protein kinase

• Park CE
• Kim MJ
• Lee JH
• Min BI
• Bae H
• Choe W
• Kim SS
• Ha J

Abstract

trans-Resveratrol (t-RVT), a naturally occurring polyphenol found in Polygonum cuspidatum, grape, and red wine, has been reported to have anti- inflammatory, cardioprotective, and cancer chemopreventive properties. However antidiabetic effect of t-RVT has not yet been reported. In this study, we show that t-RVT increases glucose uptake in C2C12 myotubes by activating AMP-activated protein kinase (AMPK), uncovering an antidiabetic potential of t-RVT for the first time. AMPK plays a central role in the regulation of glucose and lipid metabolism, and hence it is considered a novel therapeutic target for metabolic syndrome such as type 2 diabetes. t-RVT significantly induced glucose uptake in C2C12 cells, via AMPK activation, but not a phosphatidylinositol-3 kinase (PI-3 kinase) signal pathway. The induced glucose uptake was attenuated by pretreatment with a pharmacological inhibitor for AMPK, indicating that the effect of t-RVT primarily depends on AMPK activation. However, in the presence of insulin, t-RVT also potentiated the effect of insulin on glucose uptake via AMPK activation, which led to further activation of PI-3 kinase/Akt signal pathway.

MeSH Terms

1-Phosphatidylinositol 3-Kinase/metabolism
AMP-Activated Protein Kinases
Animals
Biological Transport/drug effects
Cell Line
Enzyme Activation/drug effects
Glucose/*metabolism
Insulin/metabolism
Mice
Models, Biological
Multienzyme Complexes/*metabolism
Muscle Fibers, Skeletal/*drug effects/enzymology/metabolism
Protein-Serine-Threonine Kinases/*metabolism
Proto-Oncogene Proteins c-akt/metabolism
Stilbenes/*pharmacology