Exp Mol Med.  2002 Mar;34(1):60-68.

Characterization of cis-acting elements in the rat ATP citrate-lyase gene promoter

• Moon YA
• Park SW
• Kim KS

Abstract

The cis-acting element in the promoter of the rat ATP-citrate lyase (ACL) and transcription factors which interact with these elements were determined. Six Sp1 binding sites and CAAT box exist in the region from transcription start site to -419 bp which showed the highest promoter activity in ACL promoter previously. In the region from -612 to -419, C/EBP binding site and other protein binding sites were also detected. Chloramphenicol acetyltransferase assay of ACL promoter suggested that multiple Sp1 sites might be involved in the ACL transcription. Gel mobility shift assay with antibodies against Sp1 and Sp3 revealed that DNA binding efficiency of Sp1 is increased in the liver of rats re-fed low fat/high carbohydrate diet after fasting. These results suggest that Sp1 is one of the most important transcription factors for ACL promoter to produce basal and induced transcription by low fat/high carbohydrate diet.

MeSH Terms

Adenosine Triphosphate/*metabolism
Animal
Base Sequence
CCAAT-Binding Factor/metabolism
Cells, Cultured
DNA Footprinting
Dietary Carbohydrates
Genes, Reporter
Hepatocytes/cytology
Molecular Sequence Data
Multienzyme Complexes/*genetics
Nuclear Proteins/metabolism
Oxo-Acid-Lyases/*genetics
*Promoter Regions (Genetics)
Rats
Rats, Sprague-Dawley
Transcription Factors/*metabolism
Transcription, Genetic