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Seroprevalence and Potential Risk Factors Associated with Neospora spp. Infection among Asymptomatic Horses in Jordan

Talafha AQ, Abutarbush SM, Rutley DL

This study aimed to determine the seroprevalence and to identify risk factors associated with Neospora spp. infection in horses in Jordan. Management related data were collected from each farm and...
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Review of Neospora caninum and neosporosis in animals

Dubey JP

Neospora caninum is a coccidian parasite of animals. It is a major pathogen for cattle and dogs and it occasionally causes clinical infections in horses, goats, sheep, and deer. Domestic...
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Fecal and Molecular Survey of Neospora caninum in Farm and Household Dogs in Mashhad Area, Khorasan Province, Iran

Razmi G

Neospora caninum is an important cause of abortion in dairy cattle worldwide. Dog is the definitive host for N. caninum and can infect dairy cattle. The aim of this study...
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Development of a Disperse Dye Immunoassay Technique for Detection of Antibodies against Neospora caninum in Cattle

Selahi F, Namavari M, Hosseini M, Mansourian M, Tahamtan Y

In this study a disperse dye immunoassay method was standardized and evaluated for detection of antibodies against Neospora caninum in cattle. Sera from 150 cattle with a recent history of...
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Exploration of immunoblot profiles of Neospora caninum probed with different bovine immunoglobulin classes

Shin YS, Lee EG, Jung TS

  • KMID: 1114858
  • J Vet Sci.
  • 2005 Jun;6(2):157-160.
The present study was attempted to compare the Neospora caninum (N. caninum) antigenic bands recognized by different bovine immunoglobulin classes. A total 10, 5, 2, and 6 antigenic bands were...
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Congenital Neosporosis in Goats from the State of Minas Gerais, Brazil

Varaschin , Hirsch C, Wouters F, Nakagaki K, Guimaraes , Santos DS, Bezerra PS, Costa RC, Peconick A, Langohr IM

Congenital Neospora caninum infection was diagnosed in two Saanen goat kids from two distinct herds with a history of abortion and weak newborn goat kids in the Southern region of...
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Loss of infectivity of Neospora caninum oocysts maintained for a prolonged time

Uzeda RS, Costa KS, Santos SL, Pinheiro A, Almeida MA, McAllister MM, Gondim LF

The purpose of this study was to investigate whether sporulated Neospora caninum oocysts, which had been stored for 46 mo in a 2% sulfuric acid solution at 4 degrees C,...
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ELISA detection of IgG antibody against a recombinant major surface antigen (Nc-p43) fragment of Neospora caninum in bovine sera

Ahn HJ, Kim S, Kim DY, Nam HW

An ELISA was established to measure bovine IgG directed against the recombinant antigenic determinant of Nc-p43, a major surface antigen of Neospora caninum. In a previous study, two thirds of...
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Seroprevalence of antibodies to Neospora caninum in dogs and raccoon dogs in Korea

Kim JH, Kang MS, Lee BC, Hwang WS, Lee CW, So BJ, Dubey JP, Kim DY

Neospora caninum is an important cause of abortion in cattle, and dogs are its only known definitive host. Its seroprevalence among domestic urban and rural dogs and feral raccoon dogs...
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GC/MS analysis of high-performance liquid chromatography fractions from Sophora flavescens and Torilis japonica extracts and their in vitro anti-neosporal effects on Neospora caninum

Seo HS, Kim KH, Kim DY, Park BK, Shin NS, Kim JH, Youn H

We analyzed alcoholic extracts of herbs possessing anti-neosporal activity against Neospora (N.) caninum. To identify the chemical components of Sophora (S.) flavescens and Torilis (T.) japonica associated with anti-neosporal activity,...
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Protective efficacy of vaccination with Neospora caninum multiple recombinant antigens against experimental Neospora caninum infection

Cho JH, Chung WS, Song KJ, Na BK, Kang SW, Song CY, Kim TS

Protective efficacy of vaccination with Neospora caninum multiple recombinant antigens against N. caninum infection was evaluated in vitro and in vivo. Two major immunodominant surface antigens (NcSAG1 and NcSRS2) and...
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Detection of IgG antibody against Neospora caninum in cattle in Korea

Bae JS, Kim DY, Hwang WS, Kim JH, Lee NS, Nam HW

A total of 492 cattle sera was screened by IgG-ELISA against Neospora caninum (Nc-1 strain and a Korean isolate, KBA-2) and Toxoplasma gondii. Out of 492, 113 sera (23.0%)...
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Antibody reaction of human anti-Toxoplasma gondii positive and negative sera with Neospora caninum antigens

Nam HW, Kang SW, Choi WY

Anti-Neospora caninum antibody was detected in anti-Toxoplasma gondii positive and negative human sera by ELISA, western blot and immunofluorescence assay (IFA). Twelve cases out of 172 (6.7%) Toxoplasma-positive sera...
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Generation and Immunity Testing of a Recombinant Adenovirus Expressing NcSRS2-NcGRA7 Fusion Protein of Bovine Neospora caninum

Jia LJ, Zhang SF, Qian NC, Xuan XN, Yu LZ, Zhang XM, Liu MM

Neospora caninum is the etiologic agent of bovine neosporosis, which affects the reproductive performance of cattle worldwide. The transmembrane protein, NcSRS2, and dense-granule protein, NcGRA7, were identified as protective antigens...
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Determination of antigenic domain in GST fused major surface protein (Nc-p43) of Neospora caninum

Son ES, Ahn HJ, Kim JH, Kim DY, Nam HW

The antigenic domain of the major surface protein (Nc-p43) of Neospora caninum was examined by polymerase chain reaction of its gene fragments and recombinant expression as GST fusion proteins. The...
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Flavonoids Modulate the Proliferation of Neospora caninum in Glial Cell Primary Cultures

Barbosa de Matos R, Braga-de-Souza S, Pena Seara Pitanga B, Amaral da Silva VD, Viana de Jesus EE, Morales Pinheiro A, Dias Costa MdF, dos Santos El-Bacha R, de Oliveira Ribeiro CS, Lima Costa S

Neospora caninum (Apicomplexa; Sarcocystidae) is a protozoan that causes abortion in cattle, horses, sheep, and dogs as well as neurological and dermatological diseases in dogs. In the central nervous system...
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Two-dimensional gel electrophoresis and immunoblot analysis of Neospora caninum tachyzoites

Lee EG, Kim JH, Shin YS, Shin GW, Kim YH, Kim GS, Kim DY, Jung TS, Suh MD

  • KMID: 1120070
  • J Vet Sci.
  • 2004 Jun;5(2):139-145.
Identification of expressed protein profiles and antigenic determination are some of the most challenging aspects of proteomics. Two-dimensional gel electrophoresis (2-DE) combined with immunoblot analysis were employed to study the...
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Dembo polymerase chain reaction technique for detection of bovine abortion, diarrhea, and respiratory disease complex infectious agents in potential vectors and reservoirs

Rahpaya SS, Tsuchiaka , Kishimoto , Oba M, Katayama Y, Nunomura Y, Kokawa S, Kimura T, Kobayashi , Kirino Y, Okabayashi T, Nonaka N, Mekata H, Aoki H, Shiokawa M, Umetsu M, Morita T, Hasebe A, Otsu K, Asai T, Yamaguchi , Makino S, Murata Y, Abi AJ, Omatsu T, Mizutani T

Bovine abortion, diarrhea, and respiratory disease complexes, caused by infectious agents, result in high and significant economic losses for the cattle industry. These pathogens are likely transmitted by various vectors...
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