J Vet Sci.  2013 Sep;14(3):241-248. 10.4142/jvs.2013.14.3.241.

GC/MS analysis of high-performance liquid chromatography fractions from Sophora flavescens and Torilis japonica extracts and their in vitro anti-neosporal effects on Neospora caninum

• Seo HS
• Kim KH
• Kim DY
• Park BK
• Shin NS
• Kim JH
• Youn H

• KMID: 1705553
• DOI: http://doi.org/10.4142/jvs.2013.14.3.241

Abstract

We analyzed alcoholic extracts of herbs possessing anti-neosporal activity against Neospora (N.) caninum. To identify the chemical components of Sophora (S.) flavescens and Torilis (T.) japonica associated with anti-neosporal activity, specific fractions were isolated by high-performance liquid chromatography (HPLC). In vitro activity of the fractions against N. caninum was then assessed. Gas chromatography/mass spectrometry (GC/MS) was used to identify and quantify specific anti-neosporal molecules in the herbal extracts. Almost all HPLC fractions of S. flavescens and T. japonica had higher levels of anti-neosporal activity compared to the not treated control. Active constituents of the extracts were sophoridane, furosardonin A, and tetraisopropylidene-cyclobutane in S. flavescens; 5,17-beta-dihydroxy-de-A-estra-5,7,9,14-tetraene, furanodiene, and 9,12-octadecadienoic acid (Z,Z)-(CAS,1) in T. japonica.

Keyword

GC/MS; HPLC; Neospora caninum; Sophora flavescens; Torilis japonica

MeSH Terms

Apiaceae/*chemistry
Chromatography, High Pressure Liquid
Coccidiostats/*chemistry
Fruit/chemistry
Gas Chromatography-Mass Spectrometry
Neospora/*drug effects/growth & development
Plant Extracts/*chemistry
Plant Roots/chemistry
Sophora/*chemistry
Coccidiostats
Plant Extracts

Figure

• 

  Fig. 1 HPLC fraction G1-4-1 (Sf-1) was identified as sophoridane.

• 

  Fig. 2 HPLC fraction G3-1 (Sf-3) was identified as furosardonin A.

• 

  Fig. 3 HPLC fraction G3-5 (Sf-4) was identified as tetraisopropylidene-cyclobutane.

• 

  Fig. 4 HPLC fraction G3-6-1 (Tj-1) was identified as 5,17-β-dihydroxy-de-A-estra-5,7,9,14-tetraene.

• 

  Fig. 5 HPLC fraction G4-6 (Tj-2) was identified as furanodiene.

• 

  Fig. 6 HPLC fraction G5-2 (Tj-3) was identified as 9,12-octadecadienoic acid (Z,Z)-(CAS,1).

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