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Molecular characterization of Escherichia coli O157:H7 strains isolated from different sources and geographic regions

Regua-Mangia AH, Gonzalez A, Cerqueira , Andrade JR

Escherichia (E.) coli serotype O157:H7 is a globally distributed human enteropathogen and is comprised of microorganisms with closely related genotypes. The main reservoir for this group is bovine bowels, and...
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Virulence factors in Escherichia coli isolated from calves with diarrhea in Vietnam

Nguyen TD, Vo TT, Vu-Khac H

This study was conducted to determine the prevalence and characteristics of pathogenic Escherichia (E.) coli strains from diarrheic calves in Vietnam. A total of 345 E. coli isolates obtained from...
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Molecular analysis of fluoroquinolone-resistance in Escherichia coli on the aspect of gyrase and multiple antibiotic resistance (mar) genes

Park YH, Yoo JH, Huh DH, Cho YK, Choi JH, Shin WS

We analyzed the fluoroquinolone resistance mechanism of 28 isolates of ciprofloxacin-resistant E. coli from patients who received ciprofloxacin as a regimen of a selective gut decontamination. Isolates distinctive by infrequent...
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The expression of plasmid mediated afimbrial adhesin genes in an avian septicemic Escherichia coli strain

Stehling E, Campos TA, Brocchi M, de Carvalho Azevedo VA, da Silveira WD

An Escherichia coli strain (SEPT13) isolated from the liver of a hen presenting clinical signs of septicaemia had a LD(50) of 4.0 x 10(5) CFU/ml in one-day-old chickens, expressed Ia,...
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Expression of verocytotoxic Escherichia coli antigens in tobacco seeds and evaluation of gut immunity after oral administration in mouse model

Rossi L, Di Giancamillo A, Reggi S, Domeneghini C, Baldi A, Sala V, Dell'Orto , Coddens A, Cox E, Fogher C

Verocytotoxic Escherichia (E.) coli strains are responsible for swine oedema disease, which is an enterotoxaemia that causes economic losses in the pig industry. The production of a vaccine for oral...
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Properties of GST-CALM expressed in E. coli

Kim JA, Kim SR, Jung YK, Woo SY, Seoh JY, Hong YS, Kim HL

  • KMID: 1109309
  • Exp Mol Med.
  • 2000 Jun;32(2):93-99.
Clathrin-coated vesicles (CCVs) are involved in protein and lipid trafficking between intracellular compartments in eukaryotic cells. CCVs are composed of clathrin and assembly proteins. The clathrin assembly protein lymphoid...
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Immunization effect of recombinant P27/30 protein expressed in Escherichia coli against the hard tick Haemaphysalis longicornis (Acari: Ixodidae) in rabbits

You MJ

We investigated the induction of resistance to Haemaphysalis longicornis infestation in rabbits that had been immunized with recombinant H. longicornis P27/30 protein. The success of immunological control methods is dependent...
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Factors affecting transformation efficiency of BCG with a Mycobacterium-Escherichia coli shuttle vector pYUB18 by electroporation

Cho SN, Hwang JH, Park S, Chong Y, Kim SK, Song CY, Kim JD

BCG has been one of the vehicles for multi-recombinant vaccine. However, low transformation efficiency of BCG with plasmid DNA hampered studies involving expression of foreign antigens in BCG. In an...
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Diagnostic Efficacy of a Recombinant Cysteine Protease of Spirometra erinacei Larvae for Serodiagnosis of Sparganosis

Rahman SM, Kim JH, Hong ST, Choi MH

The mature domain of a cysteine protease of Spirometra erinacei plerocercoid larva (i.e., sparganum) was expressed in Escherichia coli, and its value as an antigen for the serodiagnosis of sparganosis...
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Expression of Recombinant Porcine Interleukin-2 and Application of Its Antibody to Immunoassays

Choi IS, Yoo HS

  • KMID: 1095123
  • J Vet Sci.
  • 2002 Sep;3(3):207-212.
Interleukin-2 plays an important role in T lymphocyte proliferation and immune response regulations. In this study, porcine IL-2 cDNA was cloned from peripheral blood mononuclear cells, and recombinant porcine IL-2...
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Efficacy of VP2 protein expressed in E. coli for protection against highly virulent infectious bursal disease virus

Omar AR, Kim CL, Bejo MH, Ideris A

The ability of a heat-inactivated whole virus from a highly virulent infectious bursal disease virus (hvIBDV) and VP2 protein from hvIBDV expressed in E. coli provided protection against a hvIBDV...
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Prokaryotic expression and characterization of human AP DNA endonuclease

Oh SH, Song DW, Lee MY

The expression of major human apurinic/apyrimidinic DNA endonuclease (APEX) from its cDNA in E. coli (DH5 alpha) was attempted in order to obtain a biologically active recombinant APEX....
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Comparison of 3 Phenotypic-detection Methods for Identifying Plasmid-mediated AmpC beta-lactamase-producing Escherichia coli, Klebsiella pneumoniae, and Proteus mirabilis Strains

Lee W, Jung B, Hong SG, Song W, Jeong SH, Lee K, Kwak HS

BACKGROUND: Plasmid-mediated AmpC beta-lactamases (PABLs) have been detected in the strains of Escherichia coli, Klebsiella spp., Proteus mirabilis, and Salmonella spp. PABLs may be difficult to detect and might interfere...
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Purification and characterization of recombinant murine endostatin in E. coli

You WK, So SH, Lee H, Park SY, Yoon MR, Chang SI, Kim HK, Joe YA, Hong YK, Chung SI

  • KMID: 1029927
  • Exp Mol Med.
  • 1999 Dec;31(4):197-202.
Endostatin, a carboxyl-terminal fragment of collagen XVIII is known as an anti-angiogenic agent, that specifically inhibits the proliferation of endothelial cell and the growth of several primary tumor....
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Effects of Val34Leu and Val35Leu polymorphism on the enzyme activity of the coagulation factor XIII-A

Lee IH, Chung SI, Lee SY

  • KMID: 755212
  • Exp Mol Med.
  • 2002 Nov;34(5):385-390.
Change in fibrin stabilizing activity of factor XIII A subunit (FXIII-A) caused by a specific mutation, Val34Leu, is recently implicated to incidences of pathophysiology of thrombosis. In an effort to...
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Use of hydrophilic extra-viral domain of canine distemper virus H protein for enzyme-linked immunosorbent assay development

Cho KH, Kim J, Yoo HA, Kim DH, Park SY, Song CS, Choi IS, Lee JB

Simple methods for measuring the levels of serum antibody against canine distemper virus (CDV) would assist in the effective vaccination of dogs. To develop an enzyme-linked immunosorbent assay (ELISA) specific...
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Molecular cloning, purification and immunogenicity of recombinant Brucella abortus 544 malate dehydrogenase protein

Reyes AW, Simborio H, Hop HT, Arayan LT, Kim S

The Brucella mdh gene was successfully cloned and expressed in E. coli. The purified recombinant malate dehydrogenase protein (rMDH) was reactive to Brucella-positive bovine serum in the early stage, but...
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Gene Cloning, Expression and Immunogenicity of the Protective Antigen Subolesin in Dermacentor silvarum

Hu Y, Zeng H, Zhang J, Wang D, Li D, Zhang T, Yang S, Liu J

Subolesin (4D8), the ortholog of insect akirins, is a highly conserved protective antigen and thus has the potential for development of a broad-spectrum vaccine against ticks and mosquitoes. To date,...
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Development and Clinical Evaluation of a Rapid Serodiagnostic Test for Toxoplasmosis of Cats Using Recombinant SAG1 Antigen

Chong CK, Jeong W, Kim HY, An DJ, Jeoung HY, Ryu JE, Ko AR, Kim YJ, Hong SJ, Yang Z, Nam HW

Rapid serodiagnostic methods for Toxoplasma gondii infection in cats are urgently needed for effective control of transmission routes toward human infections. In this work, 4 recombinant T. gondii antigens (SAG1,...
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Development and characterization of a potential diagnostic monoclonal antibody against capsid protein VP1 of the chicken anemia virus

Lien YY, Huang CH, Sun FC, Sheu SC, Lu TC, Lee MS, Hsueh SC, Chen HJ, Lee MS

Chicken anemia virus (CAV) is an important viral pathogen that causes anemia and severe immunodeficiency syndrome in chickens worldwide. In this study, a potential diagnostic monoclonal antibody against the CAV...
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