J Vet Sci.  2006 Sep;7(3):241-247. 10.4142/jvs.2006.7.3.241.

Efficacy of VP2 protein expressed in E. coli for protection against highly virulent infectious bursal disease virus

• Omar AR
• Kim CL
• Bejo MH
• Ideris A

• KMID: 1089908
• DOI: http://doi.org/10.4142/jvs.2006.7.3.241

Abstract

The ability of a heat-inactivated whole virus from a highly virulent infectious bursal disease virus (hvIBDV) and VP2 protein from hvIBDV expressed in E. coli provided protection against a hvIBDV challenge in specificpathogen- free (SPF) chickens. Six out of seven chickens that were injected three times with crude VP2 protein developed significant antibody titer against IBDV. However, only four out of the seven chickens survived the hvIBDV challenge. Despite showing low antibody titer profiles, all chickens immunized with the heat-inactivated whole virus also survived the challenged with hvIBDV. However, all of these chickens had bursal atrophy and mild to moderate depletion of lymphocytes. Thus, antibodies raised against IBDV VP2 protein expressed in E. coli and denatured IBDV proteins induced some degree of protection against mortality but not against bursal damage following challenge with hvIBDV.

Keyword

antibody response; E. coli; heat-inactivated; infectious bursal disease virus; VP2 subunit vaccine

MeSH Terms

Animals
Antibodies, Viral/blood
Birnaviridae Infections/immunology/prevention & control/*veterinary/virology
Chickens
Enzyme-Linked Immunosorbent Assay/veterinary
Escherichia coli/genetics
Immunization/standards/*veterinary
Infectious bursal disease virus/genetics/*immunology/pathogenicity
Poultry Diseases/*immunology/prevention&control/virology
Recombinant Proteins/genetics/*immunology
Specific Pathogen-Free Organisms
Vaccines, Attenuated/immunology/pharmacology
Vaccines, Synthetic/immunology/pharmacology
Viral Structural Proteins/biosynthesis/genetics/*immunology
Viral Vaccines/*immunology/pharmacology

Figure

• 

  Fig. 1 Western blot analysis of VP2 protein. Lane 1, standard protein marker; lane 2, BL21-SI host cells only and lane 3, cell lysate sample of 3 h induced recombinant plasmid. Arrow indicates the evidence of ~50 kDa VP2 protein detected by rabbit polyclonal antibody against IBDV.

• 

  Fig. 2 Optimization of NaCl concentration for expression of VP2 protein in the E. coli strain BL21-SI. Lane 1, standard protein marker; lane 2, 0.1 M NaCl; lane 3, 0.2 M NaCl; lane 4, 0.3 M NaCl and lane 5, 0.4 M NaCl. Arrow indicates that the highest expression of VP2 protein was achieved with 0.3 M NaCl.

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