Korean J Parasitol.  2004 Dec;42(4):195-200. 10.3347/kjp.2004.42.4.195.

Immunization effect of recombinant P27/30 protein expressed in Escherichia coli against the hard tick Haemaphysalis longicornis (Acari: Ixodidae) in rabbits

• You MJ

• KMID: 1720489
• DOI: http://doi.org/10.3347/kjp.2004.42.4.195

Abstract

We investigated the induction of resistance to Haemaphysalis longicornis infestation in rabbits that had been immunized with recombinant H. longicornis P27/30 protein. The success of immunological control methods is dependent upon the use of potential key antigens as tick vaccine candidates. Previously, we cloned a gene encoding 27 kDa and 30 kDa proteins (P27/30) of H. longicornis, and identified P27/30 as a troponin I-like protein. In this study, rabbits that were immunized with recombinant P27/30 expressed in Escherichia coli showed the statistically significant longer feeding duration for larval and adult ticks (P< 0.05), low engorgement rates in larval ticks (64.4%), and an apparent reduction in egg weights, which suggest that H. longicornis P27/30 protein is a potential candidate antigen for a tick vaccine. These results demonstrated that the recombinant P27/30 protein might be a useful vaccine candidate antigen for biological control of H. longicornis.

Keyword

P27/30 protein; Haemaphysalis longicornis; ticks; immunity; tick control

MeSH Terms

Animals
Antibodies/blood
Escherichia coli/genetics
Female
Gene Expression
Insect Proteins/immunology
Ixodidae/*immunology
Microfilament Proteins/*immunology
Rabbits
Recombinant Proteins/*immunology
Research Support, Non-U.S. Gov't
Tick Infestations/*immunology/prevention & control