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Dot-Blot Immunoassay of Fasciola gigantica Infection using 27 kDa and Adult Worm Regurge Antigens in Egyptian Patients

Kamel HH, Saad GA, Sarhan RM

The purpose of the present study was to evaluate the potential role of the 27-Kilodalton (KDa) antigen versus Fasciola gigantica adult worm regurge antigens in a DOT-Blot assay and to...
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Identification of IgE-reacting Clonorchis sinensis antigens

Yong TS, Park SJ, Lee DH, Yang HJ, Lee J

Clonorchis sinensis is a liver fluke and it is the most prevalent human parasite in Korea at present. The parasite infection induces immune responses, characteristically an increased production...
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Antigenic profile and localization of Clonorchis sinensis proteins in the course of infection

Hong SJ, Kim TY, Song KY, Sohn WM, Kang SY

In the course of Clonorchis sinensis infection, antigens presented to the hosts may be in a close relation to growth of the fluke. The antigenic proteins stimulating IgG antibody production...
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Immunoblot Patterns of Taenia asiatica Taeniasis

Jeon HK, Eom KS

Differential diagnosis of Taenia asiatica infection from other human taeniases by serology has been tested. An enzyme-linked immunoelectrotransfer blot (EITB) was applied to subjected human sera and tapeworm materials. Thirty-eight...
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Metagonimus yokogawai: a 100-kDa Somatic Antigen Commonly Reacting with Other Trematodes

Han ET, Yang HJ, Park YJ, Park JH, Chai JY

  • KMID: 1792170
  • Korean J Parasitol.
  • 2014 Apr;52(2):201-204.
This study was undertaken to characterize the properties of a 100 kDa somatic antigen from Metagonimus yokogawai. Monoclonal antibodies (mAbs) were produced against this 100 kDa antigen, and their immunoreactivity...
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Variation of antigenic proteins of eggs and developmental stages of Paragonimus westermani

Kong Y, Chung JY, Yun DH, Kim LS, Kang SY, Ito A, Ma L, Cho SY

Diagnosis of early paragonimiasis is difficult because parasitological evidence is not easily obtained. Antibody tests have been proposed as a good substitute for classical diagnostic techniques. Using the...
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A Clonorchis sinensis-specific antigen that detects active human clonorchiasis

Kim SI

A Clonorchis sinensis-specific antigen in excretory-secretory product of C. sinensis (CsE) was assessed in human clonorchiasis by immunoblot. Thirty and 7 kDa antigens of CsE2, one of four different batches...
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Cross-reactivity of Toxocariasis with Crude Antigen of Toxascaris leonina Larvae by ELISA

Jin Y, Shen C, Huh S, Choi MH, Hong ST

Roundworms of Toxocara canis and Toxascaris leonina are common gastrointestinal helminths of canids over the world. Humans are infected with T. canis larvae through ingestion of infective eggs in contaminated...
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The 10 kDa protein of Taenia solium metacestodes shows genus specific antigenicity

Park SK, Yun DH, Chung JY, Kong Y, Cho SY

Genus specific antigenicity of the 10 kDa protein in cyst fluid (CF) of Taenia solium metacestodes was demonstrated by comparative immunoblot analysis. When CFs from taeniid metacestodes of T. saginata,...
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Immunodiagnosis of clonorchiasis using a recombinant antigen

Yong TS, Yang HJ, Park SJ, Kim YK, Lee DH, Lee SM

A cDNA expression library of Clonorchis sinensis adult worm was constructed, and screened out immunologically. One clone, pBCs31, was selected in view of its predominant reactivity with an experimentally infected...
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Intestinal Paragonimiasis with Colonic Ulcer and Hematochezia in An Elderly Taiwanese Woman

Liu CT, Chen YC, Chen TH, Barghouth , Fan CK

  • KMID: 1792129
  • Korean J Parasitol.
  • 2012 Dec;50(4):349-352.
A 94-year-old female with end-stage renal disease presents with fever, fatigue, and hematochezia. She had previously resided in Hunan Province, China, and Myanmar, and she immigrated to Taiwan 30 years...
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Tissue parasitic helminthiases are prevalent at Cheongjin, North Korea

Shen C, Li S, Zheng S, Choi MH, Bae YM, Hong ST

We investigated a small-scale serological survey to screen tissue-parasitic helminthiases of North Koreans as one of research programs for re-unification of Korea. Soil-transmitted helminthiases were found highly prevalent among North...
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Seroprevalence of Trichinella sp. in Wild Boars (Sus scrofa) from Yanggu-gun, Gangwon-do, Korea

Lee HJ, Chung OS, Kim JL, Lee SH, Yoo YB, Seo M

A total 7 outbreaks of trichinellosis have occurred in Korea, mostly as a result of consumption of raw wild boar (Sus scrofa) meat. Since only 1 serological survey on wild...
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Usefulness of 8 kDa protein of Fasciola hepatica in diagnosis of fascioliasis

Kim KS, Yang HJ, Chung YB

This study was designed to detect and evaluate an antigenicity of low molecular weight proteins of Fasciola hepatica in fascioliasis. Low molecular weight protein of F. hepatica was purified by...
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Evaluation of IgG4 Subclass Antibody Detection by Peptide-Based ELISA for the Diagnosis of Human Paragonimiasis Heterotrema

Intapan PM, Sanpool O, Janwan P, Laummaunwai P, Morakote N, Kong Y, Maleewong

A synthetic peptide was prepared based on the antigenic region of Paragonimus westermani pre-procathepsin L, and its applicability for immunodiagnosis for human paragonimiasis (due to Paragonimus heterotremus) was tested using...
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Serological and Molecular Characteristics of the First Korean Case of Echinococcus multilocularis

Jeong JS, Han SY, Kim YH, Sako Y, Yanagida T, Ito A, Chai JY

  • KMID: 1792155
  • Korean J Parasitol.
  • 2013 Oct;51(5):595-597.
In December 2011, we reported an autochthonous case of Echinococcus multilocularis infection in a 42-year-old woman in Korea. The diagnosis was based on histopathological findings of the surgically resected liver...
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Persisting antibody reaction in paragonimiasis after praziquantel treatment is elicited mainly by egg antigens

Cho SY, Kong Y, Yun DH, Kang SY, Kim LS, Chung YB, Yang HJ

Antibody responses in serum and cerebrospinal fluid (CSF) samples from patients with active and chronic paragonimiasis and in sera from patients on whom follow-up studies were done after praziquantel treatment...
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Detection of Gnathostoma spinigerum Antibodies in Sera of Non-Traumatic Subarachnoid Hemorrhage Patients in Thailand

Kitkhuandee A, Munkong W, Sawanyawisuth K, Janwan P, Maleewong , Intapan PM

Gnathostoma spinigerum can cause subarachnoid hemorrhage (SAH). The detection of specific antibodies in serum against G. spinigerum antigen is helpful for diagnosis of neurognathostomiasis. There is limited data on the...
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Monoclonal Antibody-Based Dipstick Assay: A Reliable Field Applicable Technique for Diagnosis of Schistosoma mansoni Infection Using Human Serum and Urine Samples

Demerdash , Mohamed S, Hendawy M, Rabia I, Attia M, Shaker Z, Diab TM

A field applicable diagnostic technique, the dipstick assay, was evaluated for its sensitivity and specificity in diagnosing human Schistosoma mansoni infection. A monoclonal antibody (mAb) against S. mansoni adult worm...
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A Recombinant Matrix Metalloproteinase Protein from Gnathostoma spinigerum for Serodiagnosis of Neurognathostomiasis

Janwan P, Intapan PM, Yamasaki H, Laummaunwai P, Sawanyawisuth K, Wongkham C, Tayapiwatana C, Kitkhuandee A, Lulitanond V, Nawa Y, Maleewong

Neurognathostomiasis is a severe form of human gnathostomiasis which can lead to disease and death. Diagnosis of neurognathostomiasis is made presumptively by using clinical manifestations. Immunoblotting, which recognizes antigenic components...
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