Korean J Parasitol.  1998 Sep;36(3):183-190. 10.3347/kjp.1998.36.3.183.

Immunodiagnosis of clonorchiasis using a recombinant antigen

• Yong TS
• Yang HJ
• Park SJ
• Kim YK
• Lee DH
• Lee SM

• KMID: 662562
• DOI: http://doi.org/10.3347/kjp.1998.36.3.183

Abstract

A cDNA expression library of Clonorchis sinensis adult worm was constructed, and screened out immunologically. One clone, pBCs31, was selected in view of its predominant reactivity with an experimentally infected rabbit serum. Recombinant C. sinensis antigen with 28 kDa as a beta-galactosidase fusion protein produced in Escherichia coli was identified by immunoblot analysis. The cloned gene was composed of 16 copies of a 30 base pair repeat and an additional 320 bases. The deduced amino acid sequence of the tandem repeat was AQPPKSGDGG. On RNA slot blot analysis. C. sinensis adult worm RNA showed a positive reaction with the cloned gene. Enzyme-linked immunosorbent assay using a purified recombinant antigen of pBCs31 showed high specificity for diagnosis of clonorchiasis.

MeSH Terms

Amino Acid Sequence
Animal
Antigens, Helminth*/genetics
Antigens, Helminth*/chemistry
Base Sequence
Clonorchiasis/diagnosis*V
Clonorchis sinensis/immunology*V
Clonorchis sinensis/genetics
Enzyme-Linked Immunosorbent Assay
Fishes
Gene Library
Genes, Helminth
Immunologic Tests/methods
Molecular Sequence Data
Rabbits
Recombinant Proteins
Tandem Repeat Sequences