Korean J Parasitol.  2003 Mar;41(1):35-39. 10.3347/kjp.2003.41.1.35.

Excretory-secretory antigen is better than crude antigen for the serodiagnosis of clonorchiasis by ELISA

  • 1Department of Parasitology and Tropical Medicine, and Institute of Endemic Diseases, Seoul National University College of Medicine, Seoul 110-799, Korea. hst@snu.ac.kr


Although stool examination is the standard diagnostic method of clonorchiasis, serodiagnosis by ELISA using crude antigen is now widely used because of its convenience. However, ELISA diagnosis still suffers from cross-reactions, and therefore there is a need to improve the present conventional ELISA. The present study was undertaken to evaluate the diagnostic value of ELISA using excretory-secretory antigen (ESA) instead of crude antigen (CA) of Clonorchis sinensis. The diagnostic sensitivity of ELISA using excretory-secretory antigen was 92.5%, which was higher than that of ELISA using crude Clonorchis sinensis antigen (88.2%). In addition, the specificity of excretory-secretory antigen was found 93.1% while that of crude antigen was 87.8%. In summary, Clonorchis sinensis ESA was found to be a better serodiagnostic antigen than CA for ELISA.

MeSH Terms

Antigens, Helminth/*analysis/immunology
Clonorchis sinensis/immunology
Enzyme-Linked Immunosorbent Assay/*methods
Sensitivity and Specificity
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