Korean J Parasitol.  2003 Jun;41(2):121-123. 10.3347/kjp.2003.41.2.121.

Usefulness of 8 kDa protein of Fasciola hepatica in diagnosis of fascioliasis

Affiliations
  • 1Department of General Surgery, Cheju National University Hospital, Jeju 690-716, Korea.
  • 2Department of Parasitology, College of Medicine, Ewha Womans University, Seoul 158-710, Korea.
  • 3Department of Parasitology and Institute of Medical Science, College of Medicine, Cheju National University, Jeju 690-756, Korea.

Abstract

This study was designed to detect and evaluate an antigenicity of low molecular weight proteins of Fasciola hepatica in fascioliasis. Low molecular weight protein of F. hepatica was purified by ammonium sulfate precipitation and Sephacryl S-100 HR gel filtration. The protein obtained was estimated to be 8 kDa on 7.5-15% gradient sodium dodecyl sulfate gel electrophoresis. Immunoblotting studies showed that the 8 kDa protein reacted with human fascioliasis sera, but not other trematodiasis sera. This result suggests that the 8 kDa protein of F. hepatica is one of diagnostic antigens in human fascioliasis without cross-reaction with other human trematodiasis.

Keyword

Fasciola hepatica; low molecular protein
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