Korean J Parasitol.  2013 Dec;51(6):763-766. 10.3347/kjp.2013.51.6.763.

Evaluation of IgG4 Subclass Antibody Detection by Peptide-Based ELISA for the Diagnosis of Human Paragonimiasis Heterotrema

• Intapan PM
• Sanpool O
• Janwan P
• Laummaunwai P
• Morakote N
• Kong Y
• Maleewong

• KMID: 1712236
• DOI: http://doi.org/10.3347/kjp.2013.51.6.763

Abstract

A synthetic peptide was prepared based on the antigenic region of Paragonimus westermani pre-procathepsin L, and its applicability for immunodiagnosis for human paragonimiasis (due to Paragonimus heterotremus) was tested using an ELISA to detect IgG4 antibodies in the sera of patients. Sera from other helminthiases, tuberculosis, and healthy volunteers were used as the references. This peptide-based assay system gave sensitivity, specificity, accuracy, and positive and negative predictive values of 100%, 94.6%, 96.2%, 100%, and 88.9%, respectively. Cross reactivity was frequently seen against the sera of fascioliasis (75%) and hookworm infections (50%). Since differential diagnosis between paragonimiasis and fascioliasis can be easily done by clinical presentation and fascioliasis serology, this cross reaction is not a serious problem. Sera from patients with other parasitoses (0-25%) rarely responded to this synthetic antigen. This synthetic peptide antigen seems to be useful for development of a standardized diagnostic system for paragonimiasis.

Keyword

Paragonimus heterotremus; paragonimiasis; serodiagnosis; peptide-based ELISA

MeSH Terms

Adult
Animals
Antibodies, Helminth/*blood
Antigens, Helminth/*diagnostic use
Clinical Laboratory Techniques/*methods
Cross Reactions
Enzyme-Linked Immunosorbent Assay/methods
Humans
Immunoglobulin G/*blood
Paragonimiasis/*diagnosis
Paragonimus/*immunology
Parasitology/*methods
Predictive Value of Tests
Sensitivity and Specificity
Antibodies, Helminth
Antigens, Helminth
Immunoglobulin G