Korean J Parasitol.  1999 Dec;37(4):243-248. 10.3347/kjp.1999.37.4.243.

Usefulness of IgG4 subclass antibodies for diagnosis of human clonorchiasis

• Hong ST
• Lee M
• Sung NJ
• Cho SR
• Chai JY
• Lee SH

• KMID: 1715251
• DOI: http://doi.org/10.3347/kjp.1999.37.4.243

Abstract

The present study analyzed serum IgG subclass antibody reaction to major antigenic bands of Clonorchis sinensis to investigate improvement of its serodiagnosis. Of the four subclass antibodies, IgG1 and IgG2 antibodies were produced but not specific, IgG3 antibody was least produced, and IgG4 antibody was prominent and specific. The serum IgG antibody reaction to any of 43-50, 34-37, 26-28, and 8 kDa bands was found in 65.5% of 168 egg positive cases while IgG4 antibody reaction was found in 22.0% of them. The positive rates of IgG and IgG4 antibodies were directly correlated with the intensity of infection. All of the sera from heavily infected cases over EPG 5,000 showed positive reaction for specific IgG and IgG4 antibodies. The specific serum IgG4 antibody disappeared within 6 months after treatment. The bands of 35 kDa and 67 kDa cross-reacted with IgG antibodies but not with IgG4 antibodies in sera of other trematode infections. The present findings suggest that serum IgG4 antibody reaction to 8 kDa band is specific but not sensitive. Any method to increase its sensitivity is required for improved serodiagnosis.

MeSH Terms

Animal
Antibodies, Bacterial
Clonorchiasis/diagnosis*
Clonorchis sinensis/immunology
Cross Reactions
Human
IgG/classification*
IgG/analysis
Immunoblotting
Serologic Tests