Korean J Parasitol.
2021 Jun;59(3):257-263. 10.3347/kjp.2021.59.3.257.
Evaluation of Rapid IgG4 Test for Diagnosis of Gnathostomiasis
- Affiliations
-
- 1Institute of Parasitic Diseases, School of Basic Medical Sciences and Forensic Medicine, Hangzhou Medical College, Hangzhou, P. R. China
- 2National Institute of Parasitic Diseases, Chinese Centre for Disease Control and Prevention, Shanghai, P. R. China
- 3Department of Parasitology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand
- KMID: 2517056
- DOI: http://doi.org/10.3347/kjp.2021.59.3.257
Abstract
- Human gnathostomiasis is a parasitic disease caused by Gnathostoma nematode infection. A rapid, reliable, and practical immunoassay, named dot immuno-gold filtration assay (DIGFA), was developed to supporting clinical diagnosis of gnathostomiasis. The practical tool detected anti-Gnathostoma-specific IgG4 in human serum using crude extract of third-stage larvae as antigen. The result of the test was shown by anti-human IgG4 monoclonal antibody conjugated colloidal gold. The sensitivity and specificity of the test were both 100% for detection in human sera from patients with gnathostomiasis (13/13) and from healthy negative controls (50/50), respectively. Cross-reactivity with heterogonous serum samples from patients with other helminthiases ranged from 0 (trichinosis, paragonimiasis, clonorchiasis, schistosomiasis, and cysticercosis) to 25.0% (sparganosis), with an average of 6.3% (7/112). Moreover, specific IgG4 antibodies diminished at 6 months after treatment. This study showed that DIGFA for the detection of specific IgG4 in human sera could be a promising tool for the diagnosis of gnathostomiasis and useful for evaluating therapeutic effects.
