Korean J Parasitol.
2013 Dec;51(6):751-754. 10.3347/kjp.2013.51.6.751.
A Recombinant Matrix Metalloproteinase Protein from Gnathostoma spinigerum for Serodiagnosis of Neurognathostomiasis
- Affiliations
-
- 1Research and Diagnostic Center for Emerging Infectious Diseases, Khon Kaen University, Khon Kaen 40002, Thailand. wanch_ma@kku.ac.th
- 2Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
- 3Department of Parasitology, National Institute of Infectious Diseases, Tokyo, Japan.
- 4Department of Medicine, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
- 5Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
- 6Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand.
- 7Department of Surgery, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
- 8Department of Microbiology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
- 9Research Division, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
- KMID: 1712233
- DOI: http://doi.org/10.3347/kjp.2013.51.6.751
Abstract
- Neurognathostomiasis is a severe form of human gnathostomiasis which can lead to disease and death. Diagnosis of neurognathostomiasis is made presumptively by using clinical manifestations. Immunoblotting, which recognizes antigenic components of molecular mass 21 kDa and 24 kDa in larval extracts of Gnathostoma spinigerum (Gs 21/24), has high sensitivity and specificity for diagnosis of neurognathostomiasis. However, only very small amounts of the Gs 21/24 antigens can be prepared from parasites harvested from natural or experimental animals. To overcome this problem, we recently produced a recombinant matrix metalloproteinase (rMMP) protein from G. spinigerum. In this study, we evaluated this rMMP alongside the Gs 21/24 antigens for serodiagnosis of human neurognathostomiasis. We studied sera from 40 patients from Srinagarind Hospital, Khon Kaen University, Thailand, with clinical criteria consistent with those of neurognathostomiasis, and sera from 30 healthy control adults from Thailand. All sera were tested for specific IgG antibodies against both G. spinigerum crude larval extract and rMMP protein using immunoblot analysis. The sensitivity and specificity for both antigenic preparations were all 100%. These results show that G. spinigerum rMMP protein can be used as an alternative diagnostic antigen, in place of larval extract, for serodiagnosis of neurognathostomiasis.
Keyword
MeSH Terms
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Adult
Animals
Antibodies, Helminth/blood
Antigens, Helminth/*diagnostic use/genetics/isolation & purification
Central Nervous System Parasitic Infections/*diagnosis/parasitology
Gnathostoma/enzymology/immunology/*isolation & purification
Gnathostomiasis/*diagnosis/parasitology
Healthy Volunteers
Humans
Immunoblotting/methods
Immunoglobulin G/blood
Matrix Metalloproteinases/*diagnostic use/genetics/isolation & purification
Parasitology/*methods
Prospective Studies
Recombinant Proteins/diagnostic use/genetics/isolation & purification
Sensitivity and Specificity
Serologic Tests/methods
Thailand
Antibodies, Helminth
Antigens, Helminth
Immunoglobulin G
Recombinant Proteins
Matrix Metalloproteinases
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