Korean J Intern Med.  1999 Jul;14(2):47-54.

Expression of ICAM-1 on the Hantaan virus-infected human umbilical vein endothelial cells

• Song JS
• Min CH
• Kang E
• Yu SH

Abstract

OBJECTIVES
In HFRS, there is a varying degree of disseminated intravascular coagulation which was evident in the early phase of the illness. It is believed also that DIC would be the consequence, at least in part, of functional changes of endothelium resulting in kinin activation and clinical syndrome. This study investigated the role of adhesion molecule in the pathogenesis of Hantaan virus-related disease. METHODS: The expression of ICAM-1 antigen on the cell membrane of human umbilical vein endothelial cells was assessed by immunohistochemistry, and ICAM-1 mRNA in the endothelial cells was assessed by in situ hybridization after Hantaan virus infection (2.6 x 10(4) PFU/mL) with the time course. RESULTS: In immunohistochemistry, the number of ICAM-1 positive cells increased with time during the 12 or 24 hours after infection. 5 to 10% of HUVECs had been positive after 12-24 hours and the number of positive cells decreased abruptly after 24 hours. Hantaan antigen had been noticed after 12 hours focally on the HUVECs but continued to proliferate into day 7 post-infection when most of HUVECs were infected by Hantaan virus. In situ hybridization showed identical patterns of ICAM-1 mRNA expression after Hantaan virus infection. CONCLUSION: It implies that the Hantaan virus infection on HUVECs would express more ICAM-1 on their surface and implicated in the pathogenesis of early clinical syndrome of HFRS.

Keyword

Hemorrhagic fever with renal syndrome; Hantaan virus; ICAM-1, immunohistochemistry, in situ hybridization

MeSH Terms

Cell Line
Endothelium, Vascular/virology
Endothelium, Vascular/immunology
Gene Expression
Hantaan Virus/pathogenicity*
Hemorrhagic Fever with Renal Syndrome/immunology*
Hemorrhagic Fever with Renal Syndrome/genetics
Hemorrhagic Fever with Renal Syndrome/etiology
Human
Immunohistochemistry
In Situ Hybridization
Intercellular Adhesion Molecule-1/metabolism*
Intercellular Adhesion Molecule-1/genetics*
RNA, Messenger/metabolism
RNA, Messenger/genetics