Korean J Nephrol.  2002 Sep;21(5):706-712.

ICAM-1 and VCAM-1 on Human Umbilical Vein Endothelial Cells Infected by Hantaan Virus

• Seol MJ
• Kang ET
• Lee TW
• Kim CH
• Choi JY
• Yoo SJ
• Oh DJ
• Yu SH

Abstract

PURPOSE: Hemorrhagic fever with renal syndrome (HFRS) is an acute febrile disease caused by Hantaan virus. It is believed that various kinds of inflammatory mediators and cytokines might be involved in its pathogenesis.
METHODS
We studied the expression of adhesion molecules, including ICAM-1 and VCAM-1 on Hantaan virus-infected human umbilical vein endothelial cells (HUVECs) with ELISA.
RESULTS
Hantaan antigen increased gradually 48 hours after virus infection, ICAM-1 increased rapidly 3 hours after virus infection and VCAM-1 increased rapidly 4 hours after infection, a little later than ICAM-1. The patterns of the change of reaction were similar between the different concentrations of virus solution that had been adsorbed on HUVECs, but the expression of ICAM-1 or VCAM-1 was greater when HUVECs were infected with stock virus rather than when infected with tenfold diluted virus.
CONCLUSION
We report the quantification using ELISA of ICAM-1 and VCAM-1 expression of HUVECs that have been infected by Hantaan virus. Increase of their expression would play an important role in the pathogenesis of HFRS.

Keyword

Hantaan virus; Endothelial cells; ICAM-1; VCAM-1; ELISA

MeSH Terms

Cytokines
Endothelial Cells
Enzyme-Linked Immunosorbent Assay
Hantaan virus*
Hemorrhagic Fever with Renal Syndrome
Human Umbilical Vein Endothelial Cells*
Humans*
Intercellular Adhesion Molecule-1*
Vascular Cell Adhesion Molecule-1*
Cytokines
Intercellular Adhesion Molecule-1
Vascular Cell Adhesion Molecule-1