Exp Mol Med.  2005 Apr;37(2):101-110.

Identification and characterization of peroxisome proliferator response element in the mouse GLUT2 promoter

• Im SS
• Kim JW
• Kim TH
• Song XL
• Kim SY
• Kim HI
• Ahn YH

Abstract

In the present study, we show that the expression of type 2 glucose transporter isoform (GLUT2) could be regulated by PPAR-gamma in the liver. Rosiglitazone, PPAR-gamma agonist, activated the GLUT2 mRNA level in the primary cultured hepatocytes and Alexander cells, when these cells were transfected with PPAR-gamma/RXR-alpha. We have localized the peroxisome proliferator response element in the mouse GLUT2 promoter by serial deletion studies and site-directed mutagenesis. Chromatin immunoprecipitation assay using ob/ob mice also showed that PPAR-gamma rather than PPAR-alpha binds to the -197/-184 region of GLUT2 promoter. Taken together, liver GLUT2 may be a direct target of PPAR-gamma ligand contributing to glucose transport into liver in a condition when PAPR-gamma expression is increased as in type 2 diabetes or in severe obesity.

Keyword

GLUT2; liver; promoter; PPAR-gamma; rosiglitazone; type 2 diabetes

MeSH Terms

Animals
Cells, Cultured
Chromatin Immunoprecipitation
Gene Expression Regulation
Genes, Reporter
Hepatocytes/*metabolism
Liver/metabolism
Male
Mice
Mice, Inbred ICR
Mice, Transgenic
Monosaccharide Transport Proteins/*biosynthesis/genetics
Mutagenesis, Site-Directed
PPAR alpha/genetics/metabolism
PPAR gamma/agonists/genetics/*metabolism
*Promoter Regions (Genetics)
Protein Isoforms/biosynthesis
Research Support, Non-U.S. Gov't
*Response Elements
Thiazolidinediones/pharmacology