Korean Circ J.  2020 Mar;50(3):236-247. 10.4070/kcj.2019.0195.

Novel Associations between Related Proteins and Cellular Effects of High-Density Lipoprotein

Affiliations
  • 1Cardiovascular Research Institute, Yonsei University College of Medicine, Seoul, Korea. shl1106@yuhs.ac
  • 2Center for Theragnosis, Biomedical Research Institute, Korean Institute of Science and Technology, Seoul, Korea. jelee9137@kist.re.kr
  • 3Department of Biochemistry, College of Life Science and Biotechnology, Yonsei University, Seoul, Korea.
  • 4Department of Chemistry, Sookmyung Women's University, Seoul, Korea.
  • 5Proteometech Inc., Seoul, Korea.
  • 6Division of Cardiology, Department of Internal Medicine, Severance Hospital, Yonsei University College of Medicine, Seoul, Korea.
  • 7KHU-KIST Department of Converging Science and Technology, Kyung Hee University, Seoul, Korea.
  • 8Division of Bio-Medical Science & Technology, KIST School, Korea University of Science and Technology, Seoul, Korea.

Abstract

BACKGROUND AND OBJECTIVES
Recent studies have examined the structure-function relationship of high-density lipoprotein (HDL). This study aimed to identify and rank HDL-associated proteins involved in several biological function of HDL.
METHODS
HDLs isolated from 48 participants were analyzed. Cholesterol efflux capacity, effect of HDL on nitric oxide production, and vascular cell adhesion molecule-1 expression were assessed. The relative abundance of identified proteins in the highest vs. lowest quartile was expressed using the normalized spectral abundance factor ratio.
RESULTS
After adjustment by multiple testing, six proteins, thyroxine-binding globulin, alpha-1B-glycoprotein, plasma serine protease inhibitor, vitronectin, angiotensinogen, and serum amyloid A-4, were more abundant (relative abundance ratio ≥2) in HDLs with the highest cholesterol efflux capacity. In contrast, three proteins, complement C4-A, alpha-2-macroglobulin, and immunoglobulin mu chain C region, were less abundant (relative abundance ratio <0.5). In terms of nitric oxide production and vascular cell adhesion molecule-1 expression, no proteins showed abundance ratios ≥2 or <0.5 after adjustment. Proteins correlated with the functional parameters of HDL belonged to diverse biological categories.
CONCLUSIONS
In summary, this study ranked proteins showing higher or lower abundance in HDLs with high functional capacities and newly identified multiple proteins linked to cholesterol efflux capacity.

Keyword

Atherosclerosis; Cardiovascular diseases; Proteomics; Lipoproteins; Immunity
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