Kidney Res Clin Pract.  2019 Dec;38(4):462-471. 10.23876/j.krcp.19.053.

Protective effects of D-005, a lipid extract from Acrocomia crispa fruits, against ischemia/reperfusion-induced acute kidney injury in rats

• Oyarzábal-Yera A
• Rodríguez-Salgueiro S
• Merino-García N
• Ocaña-Nápoles L
• González-Núñez L
• Mena-Valdés L
• Zamora-Rodríguez Z
• Medina-Pírez JA
• Jiménez-Despaigne S
• Molina-Cuevas V

• KMID: 2469376
• DOI: http://doi.org/10.23876/j.krcp.19.053

Abstract

BACKGROUND
Acute kidney injury (AKI) induced by renal ischemia/reperfusion (IR) is associated with enhanced production of reactive oxygen species in renal tissues. D-005, a lipid extract obtained from Acrocomia crispa fruit, has previously shown antioxidant effects. The aim of this work was to evaluate the effects of D-005 on renal IR-induced AKI in rats.
METHODS
Rats were randomized into seven groups including a negative control group (vehicle) without AKI and six groups with renal IR-induced AKI as follows: a positive control (vehicle); D-005 treatment at 25, 100, 200, or 400 mg/kg; and dexamethasone at 3 mg/kg. All treatments were orally administered as single doses 1 hour before AKI induction. Biomarkers (serum creatinine, urea, and uric acid concentrations), oxidative variables, and histopathological AKI changes were evaluated in blood and kidney tissues.
RESULTS
All D-005 doses protected against IR-induced AKI in rats by significantly decreasing biomarkers and histopathological AKI changes as assessed by reduced serum concentrations of creatinine, urea, and uric acid. In addition, all D-005 doses decreased tubular damage, as shown by fewer detached cells and casts in the tubular lumen. D-005 reversed oxidation disturbance markers by decreasing malondialdehyde and sulfhydryl group concentrations in plasma and in kidney homogenates and by increasing kidney catalase activity. Dexamethasone, the reference substance, protected against IR-induced AKI in rats by reducing biochemical and histological variables of renal damage in a similar manner.
CONCLUSION
Administration of single oral doses of D-005 markedly and significantly protected against renal IRinduced AKI, possibly due to its known antioxidant effects.

Keyword

Acrocomia crispa; Acute kidney injury; D-005; Rats; Renal ischemia/reperfusion

MeSH Terms

Acute Kidney Injury*
Animals
Antioxidants
Biomarkers
Catalase
Creatinine
Dexamethasone
Fruit*
Kidney
Malondialdehyde
Plasma
Rats*
Reactive Oxygen Species
Urea
Uric Acid
Antioxidants
Biomarkers
Catalase
Creatinine
Dexamethasone
Malondialdehyde
Reactive Oxygen Species
Urea
Uric Acid