Tissue Eng Regen Med.  2019 Dec;16(6):605-614. 10.1007/s13770-019-00210-1.

Evaluation of Polycaprolactone-Associated Human Nasal Chondrocytes as a Therapeutic Agent for Cartilage Repair

Affiliations
  • 1Department of Otolaryngology-Head and Neck Surgery, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, 222 Banpo-daero, Seocho-gu, Seoul 06591, Republic of Korea. kswent@catholic.ac.kr
  • 2Institute of Clinical Medicine Research, College of Medicine, The Catholic University of Korea, 222 Banpo-daero, Seochogu, Seoul 06591, Republic of Korea.
  • 3Department of Otolaryngology-Head and Neck Surgery, Bucheon St. Mary's Hospital, College of Medicine, The Catholic University of Korea, 327 Sosa-ro, Bucheon-si, Gyeonggi-do 14647, Republic of Korea. yellobird@catholic.ac.kr

Abstract

BACKGROUND
In this study, we manufactured a complex of human nasal septal cartilage (hNC) with polycaprolactone (PCL) for transplantation into cartilaginous skeletal defects and evaluated their characteristics.
METHODS
Nasal septum tissue was obtained from five patients aged ≥ 20 years who were undergoing septoplasty. hNCs were isolated and subcultured for three passages in vitro. To formulate the cell-PCL complex, we used type I collagen as an adhesive between chondrocyte and PCL. Immunofluorescence staining, cell viability and growth in the hNC-PCL complex, and mycoplasma contamination were assessed.
RESULTS
hNCs in PCL showed viability ≥ 70% and remained at these levels for 9 h of incubation at 4 ℃. Immunostaining of the hNC-PCL complex also showed high expression levels of chondrocyte-specific protein, COL2A1, SOX9, and aggrecan during 24 h of clinically applicable conditions.
CONCLUSION
The hNC-PCL complex may be a valuable therapeutic agent for implantation into injured cartilage tissue, and can be used clinically to repair cartilaginous skeletal defects. From a clinical perspective, it is important to set the short duration of the implantation process to achieve effective functional implantation.

Keyword

Chondrocyte; Collagen; Human nasal septum; Tissue engineering; Cartilage

MeSH Terms

Adhesives
Aggrecans
Cartilage*
Cell Survival
Chondrocytes*
Collagen
Collagen Type I
Fluorescent Antibody Technique
Humans*
In Vitro Techniques
Mycoplasma
Nasal Septum
Tissue Engineering
Adhesives
Aggrecans
Collagen
Collagen Type I
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