Anat Cell Biol.  2019 Dec;52(4):455-461. 10.5115/acb.19.144.

Immunohistochemical localization of nerve injury-induced protein-1 in mouse tissues

• Ekanayake
• Ahn M
• Kim J
• Choi Y
• Shin T

• KMID: 2466699
• DOI: http://doi.org/10.5115/acb.19.144

Abstract

Nerve injury-induced protein (Ninjurin)-1 is a cell adhesion molecule that is upregulated in neurons and Schwann cells after transection injury in rats. In this study, we investigated the localization of Ninjurin-1 in various tissues, including the cerebrum, sciatic nerve, spleen, lung, stomach, ileum, colon, liver, pancreas, kidney, testis, and skin in C57BL/6 mice, using Western blotting and immunohistochemistry. Western blot analysis showed that Ninjurin-1 was differentially expressed among organs. Ninjurin-1 was abundant in skin and ileum, weakly expressed in cerebrum, and moderately expressed in the other organs studied. Immunohistochemical analysis largely confirmed the results of the western blot analysis with often localization of Ninjurin-1 in the regions with abundant connective tissues. In addition, Ninjurin-1 was differentially expressed in various cell types in the tissues under the investigation. These findings suggest that Ninjurin-1 may play organ-specific roles in development and homeostasis of many organs.

Keyword

Mouse; Ninjurin-1; Organ

MeSH Terms

Animals
Blotting, Western
Cell Adhesion
Cerebrum
Colon
Connective Tissue
Homeostasis
Ileum
Immunohistochemistry
Kidney
Liver
Lung
Mice*
Neurons
Pancreas
Rats
Schwann Cells
Sciatic Nerve
Skin
Spleen
Stomach
Testis

Figure

• 

  Fig. 1 Western blot analysis of Ninjurin-1 expression in various mouse tissues. (A) Representative photographs of a Western blotting of Ninjurin-1 (22 kDa) and β-actin (45 kDa) in the cerebrum, sciatic nerve, spleen, lung, liver, pancreas, kidney, testis, skin, stomach, ileum, and colon. (B) Bar graphs show the expression level of Ninjurin-1 in various tissues. The optical density (OD/mm2) of the cerebrum was arbitrarily set to 1 in each blot. Data (means±standard error) are from four independent experiments.

• 

  Fig. 2 Immunohistochemical localization of Ninjurin-1 in the cerebrum (A), sciatic nerve (B), spleen (C), lung (D), stomach (E), ileum (F), colon (G), liver (H), pancreas (I), kidney ( J), testis (K), and skin (L). The slides were counterstained with hematoxylin. Arrowheads indicated the Ninjurin-1-positive immunoreaction in various tissues. Scale bars=20 µm (A–L).

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