Biomol Ther.  2019 Nov;27(6):540-552. 10.4062/biomolther.2019.027.

Alyssin and Iberin in Cruciferous Vegetables Exert Anticancer Activity in HepG2 by Increasing Intracellular Reactive Oxygen Species and Tubulin Depolymerization

Affiliations
  • 1Research and Development in Pharmaceuticals Program, Graduate School, Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand.
  • 2Division of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand. natthida@kku.ac.th
  • 3Human High Performance and Health Promotion Research Institute (HHP&HP), Khon Kaen University, Khon Kaen 40002, Thailand.
  • 4Synchrotron Light Research Institute (Public Organization), Nakhon Ratchasima 30000, Thailand.

Abstract

To determine the chemopreventive potential of alyssin and iberin, the in vitro anticancer activities and molecular targets of isothiocyanates (ITCs) were measured and compared to sulforaphane in hepatocellular carcinoma cell HepG2. The SR-FTIR spectra observed a similar pattern vis-à-vis the biomolecular alteration amongst the ITCs-treated cells suggesting a similar mode of action. All of the ITCs in this study cause cancer cell death through both apoptosis and necrosis in concentration dependent manner (20-80 μM). We found no interactions of any of the ITCs studied with DNA. Notwithstanding, all of the ITCs studied increased intracellular reactive oxygen species (ROS) and suppressed tubulin polymerization, which led to cell-cycle arrest in the S and G₂/M phase. Alyssin possessed the most potent anticancer ability; possibly due to its ability to increase intracellular ROS rather than tubulin depolymerization. Nevertheless, the structural influence of alkyl chain length on anticancer capabilities of ITCs remains inconclusive. The results of this study indicate an optional, potent ITC (viz., alyssin) because of its underlying mechanisms against hepatic cancer. As a consequence, further selection and development of effective chemotherapeutic ITCs is recommended.

Keyword

Isothiocyanates; Iberin; Alyssin; Reactive oxygen species; Tubulin depolymerization; HepG2

MeSH Terms

Apoptosis
Carcinoma, Hepatocellular
Cell Death
DNA
In Vitro Techniques
Isothiocyanates
Liver Neoplasms
Necrosis
Polymerization
Polymers
Reactive Oxygen Species*
Tubulin*
Vegetables*
DNA
Isothiocyanates
Polymers
Reactive Oxygen Species
Tubulin
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