J Obes Metab Syndr.  2019 Sep;28(3):175-185. 10.7570/jomes.2019.28.3.175.

Effect of a Single Bout of Exercise on Autophagy Regulation in Skeletal Muscle of High-Fat High-Sucrose Diet-Fed Mice

Affiliations
  • 1Health and Exercise Science Laboratory, Institute of Sports Science, Seoul National University, Seoul, Korea. songw3@snu.ac.kr
  • 2Department of Food and Nutrition, Seoul National University, Seoul, Korea.
  • 3Korea Mouse Phenotyping Center, Seoul National University, Seoul, Korea.
  • 4Laboratory of Developmental Biology and Genomics, Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, Seoul, Korea.
  • 5Institute of Sports Biology, Shaanxi Normal University, Xi’an, China.
  • 6Institute on Aging, Seoul National University, Seoul, Korea.

Abstract

BACKGROUND
Autophagy maintains metabolic homeostasis of muscles, and its impairment may cause muscle dysfunction. Exercise can improve muscle dysfunction induced by long-term high-fat diet. This study aimed to explore the association of autophagy with impaired muscle dysfunction in obese conditions and investigate its relationship with exercise-induced muscle function improvement.
METHODS
Male C57BL/6 mice (n=24) were randomly assigned to four groups: low-fat diet+plain water feeding sedentary (CON) group, low-fat diet+plain water feeding exercise (CON+EX) group, high-fat high-sucrose (HFHS) diet-fed sedentary group, and HFHS diet-fed exercise (HFHS+EX) group, and subjected to a single bout of exhaustive exercise.
RESULTS
HFHS diet resulted in shorter hanging time, reduced grip force, and lower exhaustion time and distance, and decreased lean mass per body weight. Moreover, in the soleus, which is chosen as a representative red (oxidative) muscle, LC3II/LC3I ratio, P62, and Bnip3 levels were altered following the HFHS diet, and were negatively correlated with muscle performance parameters; exercise significantly decreased the LC3II/LC3 ratio while P62 increased with HFHS diet. Autophagy-related protein changes were not found in the white (glycolytic) gastrocnemius.
CONCLUSION
The study revealed that 20-week HFHS diet causes a significant increase in body weight and fat mass, along with a decrease in muscle function. Autophagy-related LC3 and P62 protein expression was negatively correlated with muscle function, and they were reduced when a single bout of exercise stimulated the soleus of obese mice. However, no change of autophagy-related proteins was seen in the gastrocnemius.

Keyword

Acute exercise; High-fat diet; Sucrose; Autophagy; Muscle; Obesity

MeSH Terms

Animals
Autophagy*
Body Weight
Diet
Diet, High-Fat
Hand Strength
Homeostasis
Humans
Male
Mice*
Mice, Obese
Muscle, Skeletal*
Muscles
Obesity
Sucrose
Water
Sucrose
Water
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