Nutr Res Pract.  2019 Jun;13(3):205-213. 10.4162/nrp.2019.13.3.205.

Supplementation with psyllium seed husk reduces myocardial damage in a rat model of ischemia/reperfusion

Affiliations
  • 1Department of Biochemistry, School of Medicine, Catholic University of Daegu, 33 Duryugongwon-ro 17-gil, Nam-gu, Daegu 42472, Republic of Korea. leejw@cu.ac.kr

Abstract

BACKGROUND/OBJECTIVES
Myocardial infarction (MI) is caused by extensive myocardial damage attributed to the occlusion of coronary arteries. Our previous study in a rat model of ischemia/reperfusion (I/R) demonstrated that administration of arabinoxylan (AX), comprising arabinose and xylose, protects against myocardial injury. In this study, we undertook to investigate whether psyllium seed husk (PSH), a safe dietary fiber containing a high level of AX (> 50%), also imparts protection against myocardial injury in the same rat model.
MATERIALS/METHODS
Rats were fed diets supplemented with PSH (1, 10, or 100 mg/kg/d) for 3 d. The rats were then subjected to 30 min ischemia through ligation of the left anterior descending coronary artery, followed by 3 h reperfusion through release of the ligation. The hearts were harvested and cut into four slices. To assess infarct size (IS), an index representing heart damage, the slices were stained with 2,3,5-triphenyltetrazolium chloride (TTC). To elucidate underlying mechanisms, Western blotting was performed for the slices.
RESULTS
Supplementation with 10 or 100 mg/kg/d of PSH significantly reduces the IS. PSH supplementation (100 mg/kg/d) tends to reduce caspase-3 generation and increase BCL-2/BAX ratio. PSH supplementation also upregulates the expression of nuclear factor erythroid 2-related factor 2 (NRF2), and its target genes including antioxidant enzymes such as glutathione S-transferase mu 2 (GSTM2) and superoxide dismutase 2 (SOD2). PSH supplementation upregulates some sirtuins (NAD+-dependent deacetylases) including SIRT5 (a mitochondrial sirtuin) and SIRT6 and SIRT7 (nuclear sirtuins). Finally, PSH supplementation upregulates the expression of protein kinase A (PKA), and increases phosphorylated cAMP response element-binding protein (CREB) (pCREB), a target protein of PKA.
CONCLUSIONS
The results from this study indicate that PSH consumption reduces myocardial I/R injury in rats by inhibiting the apoptotic cascades through modulation of gene expression of several genes located upstream of apoptosis. Therefore, we believe that PSH can be developed as a functional food that would be beneficial in the prevention of MI.

Keyword

Infarction; apoptosis; sirtuin; functional food

MeSH Terms

Animals
Apoptosis
Arabinose
Blotting, Western
Caspase 3
Coronary Vessels
Cyclic AMP Response Element-Binding Protein
Cyclic AMP-Dependent Protein Kinases
Diet
Dietary Fiber
Functional Food
Gene Expression
Glutathione Transferase
Heart
Infarction
Ischemia
Ligation
Models, Animal*
Myocardial Infarction
Psyllium*
Rats*
Reperfusion
Sirtuins
Superoxide Dismutase
Xylose
Arabinose
Caspase 3
Cyclic AMP Response Element-Binding Protein
Cyclic AMP-Dependent Protein Kinases
Glutathione Transferase
Psyllium
Sirtuins
Superoxide Dismutase
Xylose
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