Korean Circ J.  2012 Sep;42(9):600-605. 10.4070/kcj.2012.42.9.600.

Cardiodynamics and Infarct Size in Regional and Global Ischemic Isolated Heart Model: Comparison of 1 Hour and 2 Hours Reperfusion

• Kim JH
• Kim J
• Park YH
• Chun KJ
• Kim JS
• Jang YH
• Lee MY
• Xu Z

• KMID: 2297890
• DOI: http://doi.org/10.4070/kcj.2012.42.9.600

Abstract

BACKGROUND AND OBJECTIVES
We investigated whether 1 hour reperfusion is enough to assess cardiodynamics and infarct size in both regional ischemia (RI) and global ischemia (GI) in isolated rat heart models.
MATERIALS AND METHODS
Hearts were randomly assigned to one of the following groups (each n=14): 1) Sham hearts for 1 hour; 2) Sham hearts for 2 hours; 3) 30 minutes RI followed by 1 hour reperfusion; 4) 30 minutes of RI followed by 2 hours reperfusion; 5) 30 minutes GI followed by 1 hour reperfusion; and 6) 30 minutes GI followed by 2 hours reperfusion.
RESULTS
There were no significant differences in infarct size between 1 hour and 2 hours reperfusion in both RI and GI. Left ventricular developed pressure was significantly decreased at both 1 hour and 2 hours reperfusion in groups of RI and GI compared to baseline (p<0.01). Rate-pressure product and +dP/dtmax also significantly decreased compared to baseline level at both 1 hour and 2 hours reperfusion in groups of RI and GI (p<0.05).
CONCLUSION
There was no significant difference in infarct size between 1 hour and 2 hours reperfusion in groups of RI and GI. Cardiodynamic variables measured at 1 hour and 2 hours reperfusion significantly decreased compared to baseline level. Our data suggests that reperfusion of 1 hour is sufficient to assess cardiodynamics in both regional and global ischemic isolated hearts model.

Keyword

Heart; Myocardial ischemia; Myocardial infarction; Myocardial reperfusion

MeSH Terms

Animals
Heart
Ischemia
Myocardial Infarction
Myocardial Ischemia
Myocardial Reperfusion
Rats
Reperfusion
Salicylamides
Salicylamides

Figure

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  Fig. 1 Experimental protocols. For measurement of infarct size and cardiodynamics, isolated rat hearts were exposed to 30 minutes ischemia followed by 2 hours reperfusion. Details for S1, S2, R1, R2, G1, and G2 are provided in the text.

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  Fig. 2 Morphometric changes after myocardial ischemia and reperfusion in isolated rat heart. A: representative sequential left ventricle slices each group showing area of necrosis (pale area) with triphenyltetrazolium chloride staining. B: percent of infarct area over area at risk. Values are mean±SEM. Details for S1, S2, R1, R2, G1, and G2 are provided in the text. There were no significant differences in AN/LV and AN/AR between S1 and S2, R1 and R2, and G1 and G2, respectively. AR: area at risk, AN: area of necrosis, LV: left ventricle, SEM: standard error of mean.

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  Fig. 3 Changes in coronary flow in isolated rat hearts. 1 hour and 2 hours means 1 hour and 2 hours after reperfusion, respectively. *p<0.05 vs. baseline, †p<0.05 vs. 1 hour after reperfusion.

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  Fig. 4 Changes in cardiodynamics in isolated rat hearts. 1 hour and 2 hours means 1 hour and 2 hours after reperfusion, respectively. *p<0.05 vs. baseline, †p<0.05 vs. 1 hour after reperfusion.

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