Nat Prod Sci.  2019 Jun;25(2):111-114. 10.20307/nps.2019.25.2.111.

Simultaneous Analysis of Four Standards of The Herbal Formula, DF-02, of Ephedra intermedia and Rheum palmatum, using by High Performance Liquid Chromatography-Ultraviolet Detector (HPLC-UVD)

• Choi SY
• Jeong B
• Jang HS
• Lee J
• Kwon YS
• Yoon Y
• Shin SS
• Yang H

• KMID: 2452779
• DOI: http://doi.org/10.20307/nps.2019.25.2.111

Abstract

The herbal formula, DF-02, consisting of Ephedra intermedia and Rheum palmatum are used for the treatment of the metabolic diseases such as obesity and liver fibrosis in Korean local clinics. We aimed to develop the simultaneous analytical conditions for four standards, (+)-pseudoephedrine (PSEP) and (−)-ephedrine (EP) for E. intermedia, and aloe-emodin (AE) and chrysophanol (CP) for R. palmatum using HPLC-UV techniques. The validated conditions yielded the high precision (relative standard deviation (RSD) < 3.65%) and the recoveries (94 - 106%) using the calibration curves with high linearity (R²> 0.9994). As a result, four standards of DF-02 were simultaneously determined under the developed method, which will be utilized for the quality control or evaluation of DF-02 and many herbal preparations containing E. intermedia and R. palmatum.

Keyword

Ephedra intermedia; Rheum palmatum; (+)-pseudoephedrine; (−)-ephedrine; aloe-emodin; chrysophanol; HPLC; validation

MeSH Terms

Calibration
Chromatography, High Pressure Liquid
Ephedra*
Liver Cirrhosis
Metabolic Diseases
Methods
Obesity
Plant Preparations
Quality Control
Rheum*
Plant Preparations

Figure

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  Fig. 1 The structures of (+)-pseudoephedrine (1), (−)-ephedrine (2), aloe-emodin (3) and chrysophanol (4).

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  Fig. 2 HPLC chromatogram of DF-02 formula and the standards obtained at UV 210 (A) and 254 (B) nm: (a) DF-02 formula (20 mg/mL), (b) (+)-pseudoephedrine, (c) (−)-ephedrine, (d) aloe-emodin and (e) chrysophanol. Detailed experimental conditions are described in the Experimental section.

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