Nat Prod Sci.  2018 Jun;24(2):109-114. 10.20307/nps.2018.24.2.109.

Simultaneous Determination of Four Compounds from Artemisia capillaris using High Performance Liquid Chromatography-Ultraviolet Detector (HPLC-UVD) and Their Quantitative Study in Artemisia Genus

  • 1College of Pharmacy, Kangwon National University, Chuncheon 24341, Republic of Korea.
  • 2R&D Center, Radiant Ltd., Chuncheon 24398, Republic of Korea.
  • 3College of Pharmacy, Chungnam National University, Daejeon 34134, Republic of Korea.


Artemisia capillaris Thunb. (Compositae) is a native herb of East Asian countries and has used for the treatment of jaundice, high liver fever, and digestive diseases for a long time, as well as being developed as the source of herbal preparations until now. The major components from A. capillaris were chlorogenic acid (1) and its derivatives substituted with caffeoyl moieties, such as 3,5-dicaffeoylquinic acid (2) and 4,5-dicaffeoylquinic acid (3), and coumarins, such as scoparone. In the study, four compounds, chlorogenic acid, 3,5-dicaffeoylquinic acid, 4,5-dicaffeoylquinic acid and scoparone (4) in the 70% ethanolic extract of A. capillaris were simultaneously determined by using HPLC-UVD system. This method was validated with the terms of linearity, precious and accuracy according to ICH guidelines. The developed method was successfully applied for the quantitative analysis of Artemisia genus, A. capillaris, A. iwayomogi, A. princeps, and A. argyi, distributed in Korea.


Artemisia capillaris; Artemisia iwayomogi; Artemisia princeps; Artemisia argyi; Scoparone; HPLC-UV

MeSH Terms

Asian Continental Ancestry Group
Chlorogenic Acid
Plant Preparations
Chlorogenic Acid
Plant Preparations


  • Fig. 1. Structures of four standards, chlorogenic acid (1), 3,5–dicaffeoylquinic acid (2), 4,5–dicaffeoylquinic acid (3) and scoparone (4), from A. capillaris.

  • Fig. 2. HPLC chromatogram of four standards, chlorogenic acid (1), 3,5–dicaffeoylquinic acid (2), 4,5–dicaffeoylquinic acid (3), scoparone (4), and the 70% ethanolic extract of A. capillaris (5).

  • Fig. 3. UV spectra of four standards, chlorogenic acid (1), 3,5–dicaffeoylquinic acid (2), 4,5–dicaffeoylquinic acid (3) and scoparone (4).

  • Fig. 4. Boxplot of the content of four standards in thirty-one Artemisia genus samples, chlorogenic acid (1), 3,5–dicaffeoylquinic acid (2), 4,5–dicaffeoylquinic acid (3) and scoparone (4).



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