Nat Prod Sci.  2021 Dec;27(4):280-283. 10.20307/nps.2021.27.4.280.

Simultaneous Determination of Four Compounds from Cercidiphyllum japonicum Using HPLC-UV Analysis

• Kang J
• Jang HS
• Kim JY
• Lee MS
• Bae YS
• Kwon Y
• Yang H

• KMID: 2524110
• DOI: http://doi.org/10.20307/nps.2021.27.4.280

Abstract

Cercidiphyllum japonicum is being used for the treatment of obesity and liver fibrosis in Korean local clinics. In the present study, we tried to develop an analytical methodology for the determination of the chemical markers of Cercidiphyllum japonicum. Four chemicals, maltol (1), chlorogenic acid (2), quercetin (3), and avicularin (4), were selected for method validation, and the analytical conditions were optimized and validated using high-performance liquid chromatography coupled with an ultraviolet detector (HPLC-UV). Additionally, the seasonal variations of four markers were monitored every month for six months. The contents of four chemicals markers were most detected in a sample collected in June.

Keyword

Cercidiphyllum japonicum; method validation; maltol; seasonal variation