Nat Prod Sci.  2016 Dec;22(4):238-245. 10.20307/nps.2016.22.4.238.

Simultaneous Determination of 11 Marker Compounds in Gumiganghwal-tang by HPLC-DAD and LC-MS

• Weon JB
• Jung YS
• Ryu G
• Yang WS
• Ma CJ

• KMID: 2366690
• DOI: http://doi.org/10.20307/nps.2016.22.4.238

Abstract

Gumiganghwal-tang has been used for the treatment of common cold for a long-time. We developed an accurate and sensitive high performance liquid chromatography-diode array detection (HPLC-DAD) and electrospray ionization mass spectrometry method for the simultaneous determination of ferulic acid, baicalin, bergapten, methyl eugenol, glycyrrhizin, oxypeucedanin, wogonin, nodakenin, atractylenolide III, imperatorin, and atractylenolide I in Gumiganghwal-tang samples. The analytes were separated on a Shiseido C18 column (5 µm, 4.6 mm I.D. × 250 mm) with gradient elution with acetonitrile and 0.1% trifluoroacetic acid. Eleven compounds were quantitatively determined by HPLC-DAD and identified by LC-MS data. We also validated this method. The calibration curves of all the compounds showed good linear regression. The limits of detection and the limits of quantification ranged from 0.04 to 0.63 and from 0.12 to 1.92 µg/mL, respectively. The relative standard deviation values of intra- and inter-days of this method represented less than 2.9%. The recoveries were found to be in the range of 90.06 - 107.66%. The developed method has been successfully applied to the analysis of Gumiganghwal-tang samples. The established HPLC method could be used to quality control of Gumiganghwal-tang.

Keyword

Simultaneous determination; Gumiganghwal-tang; HPLC-DAD; LC-MS

MeSH Terms

Calibration
Chromatography, High Pressure Liquid
Common Cold
Eugenol
Glycyrrhizic Acid
Limit of Detection
Linear Models
Methods
Quality Control
Spectrometry, Mass, Electrospray Ionization
Trifluoroacetic Acid
Eugenol
Glycyrrhizic Acid
Trifluoroacetic Acid

Figure

• 

  Fig. 1. Chemical structures of the 11 standard compounds of Gumiganghwal-tang.

• 

  Fig. 2. UV wavelength of the 11 standard compounds of Gumiganghwal-tang.

• 

  Fig. 3. HPLC chromatogram of standard compounds mixture (A) and Gumiganghwal -tang sample (B). Peaks: (1) ferulic acid, (2) nodakenin, (3) baicalin, (4) bergapten, (5) glycyrrhizin, (6) oxypeucedanin, (7) wogonin, (8) methyl eugenol, (9) atractylenolide III, (10) imperatorin and (11) atractylenolide I.

• 

  Fig. 4. SIM chromatograms and product ion scan spectra of the 11 marker compounds of Gumiganghwal-tang. Numbers: (1) ferulic acid, (2) nodakenin, (3) baicalin, (4) bergapten, (5) glycyrrhizin, (6) oxypeucedanin, (7) wogonin, (8) methyl eugenol, (9) atractylenolide III, (10) imperatorin and (11) atractylenolide I.

• 

Reference

References

(1). Jiang W. Y.Trends Pharmacol. Sci. 2005. 26:558–563.

(2). Liang Y. Z.., Xie P. J.Chromatogr. B Analyt. Technol. Biomed. Life Sci. 2004. 812:53–70.

(3). Bent S. J.Gen. Intern. Med. 2008. 23:854–859.

(4). Firenzuoli F.., Gori L.Evid. Based Complement. Alternat. Med. 2007. 4:37–40.

(5). Moon Y. H.., Go J. J.., Park J. Y.Kor. J. Pharmacogn. 1999. 30:18–24.

(6). Kim S. J.., Jeong H. J.., Moon P. D.., Lee K. M.., Lee H. B.., Jung H. J.., Jung S. K.., Rhee H. K.., Yang D. C.., Hong S. H.., Kim H. M.Biol. Pharm. Bull. 2005. 28:233–237.

(7). Lee M. Y.., Seo C. S.., Shin I. S.., Ha H.., Kim J. H.., Cho J. W.., Huh J. I.., Shin H. K.Regul. Toxicol. Pharmacol. 2012. 62:553–560.

(8). Jung H. W.., Mahesh R.., Park J. H.., Boo Y. C.., Park K. M.., Park Y. K.Int. Immunopharmacol. 2010. 10:155–162.

(9). Jung H. W.., Jung J. K.., Park Y. K.AsianPac. J. Allergy Immunol. 2011. 29:338–348.

(10). Kang T. J.., Lee S. Y.., Singh R. P.., Agarwal R.., Yim D. S.Acta. Oncol. 2009. 48:895–900.

(11). Okuyama E.., Hasegawa T.., Matsushita T.., Fujimoto H.., Ishibashi M.., Yamazaki M.Chem. Pharm. Bull. 2001. 49:154–160.

(12). Chin Y. W.., Jung Y. H.., Chae H. S.., Yoon K. D.., Kim J.Bull. Korean Chem. Soc. 2011. 32:2132–2134.

(13). Li S.., Han Q.., Qiao C.., Song J.., Lung Cheng C.., Xu H.Chin. Med. 2008. 3:DOI: 10.1186/1749-8546-3-7.

(14). Choi D. W.., Kim J. H.., Cho S. Y.., Kim D. H.., Chang S. Y.Toxicology. 2002. 181–182:581–586.

(15). Lin S. J.., Tseng H. H.., Wen K. C.., Suen T. T. J.Chromatogr. A. 1996. 730:17–23.

(16). Liu Q.., Zhao J.., Yan L.., Yi J.., Song J.Zhongguo Zhong Yao Za Zhi. 2010. 35:708–710.

(17). Zuo F.., Zhou Z. M.., Liu M. L.Biol. Pharm. Bull. 2001. 24:693–697.

(18). Jiang P.., Shi R.., Wang Q.., Ma Y. M.., Cui H. Y.., Liu P.., Liu C. H.Biomed. Chromatogr. 2013. 27:874–881.

(19). Samanidou V.., Tsagiannidis A.., Sarakatsianos I. J.Sep. Sci. 2012. 35:608–615.

(20). Sun Q.., Chang L.., Ren Y.., Cao L.., Sun Y.., Du Y.., Shi X.., Wang Q.., Zhang L. J.Sep. Sci. 2012. 35:2897–2907.

(21). Weon J. B.., Yang H. J.., Ma J. Y.., Ma C. J. J.Nat. Med. 2012. 66:510–515.

(22). Shin I. S.., Seo C. S.., Lee M. Y.., Ha H. K.., Huh J. I.., Shin H. K. J.Ethnopharmacol. 2012. 141:350–356.