Nat Prod Sci.  2018 Sep;24(3):206-212. 10.20307/nps.2018.24.3.206.

Simultaneous Determination of the Seven Phenylpropanoids in Xanthii Fructus Using a HPLC-PDA and LC-MS

• Seo CS
• Shin HK

• KMID: 2422014
• DOI: http://doi.org/10.20307/nps.2018.24.3.206

Abstract

Xanthii Fructus has been traditionally used for the treatment of rhinitis, rheumatoid arthritis, and eczema. In this study, a high-performance liquid chromatography-photodiode array (HPLC-PDA) method was developed and then used for the simultaneous analysis of eight phenylpropanoids in Xanthii Fructus. The analytical column used for this separation was a SunFireâ„¢ C₁₈ column, maintained at 40℃. The mobile phase used was 1.0% acetic acid in distilled water and 1.0% acetic acid in acetonitrile with gradient elution. For identify of each component, the mass spectrometer (MS) was used a Waters triple quadrupole mass spectrometer requipped with electrospray ionization (ESI) source. The HPLC-PDA method showed good linearity: correlation coefficients were ≥ 0.9996. The limits of detection and quantification of the eight compounds were 0.02 - 0.04 and 0.06 - 0.14 µg/mL, respectively. The extraction recoveries ranged from 97.51 to 108.67%. The relative standard deviation values of intra- and inter-day precision were 0.06 - 1.55 and 0.09 - 1.68%, respectively. The validated HPLC-PDA method was applied to simultaneously analyse the amounts of eight phenlypropanoids in Xanthii Fructus.

Keyword

Simultaneous; Determination; Xanthii Fructus; Phenylpropanoids; HPLC-PDA; LC-MS

MeSH Terms

Acetic Acid
Arthritis, Rheumatoid
Eczema
Limit of Detection
Methods
Rhinitis
Water
Acetic Acid
Water

Figure

• 

  Fig. 1 Chemical structures of eight marker compounds in XF.

• 

  Fig. 2 HPLC chromatograms of the standard solutions (A) and XF extract (B) at 325 nm. Identified compounds are (1) 3-CQA; (2) CA; (3) 1,4-DCQA; (4) 1,3-DCQA; (5) FA; (6) 1,5-DCQA; (7) 3,5-DCQA; and (8) 4,5-DCQA.

• 

  Fig. 3 Mass spectra of eight reference standard compounds. (A) 3-CQA; (B) CA; (C) 1,4-DCQA; (D) 1,3-DCQA; (E) FA; (F) 1,5-DCQA; (G) 3,5-DCQA; and (H) 4,5-DCQA.

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