Nat Prod Sci.  2019 Sep;25(3):275-283. 10.20307/nps.2019.25.3.275.

Development of Analytical Method and Validation using HPLC/PDA for Discrimination between Artemisiae Argyi Folium and Artemisiae Iwayomogii Herba

• Le DD
• Nguyen DH
• Zhao BT
• Min BS
• Woo MH

• KMID: 2459972
• DOI: http://doi.org/10.20307/nps.2019.25.3.275

Abstract

In this study, we described the new developed method to simultaneously discriminate two herbal drugs of Artemisiae Argyi Folium and Artemisiae Iwayomogii Herba using eight marker compounds (1 - 8) on an HPLC-PDA system. The developed method was applied to quantify the major components of two herbal drugs. The pattern analysis successfully discriminated and evaluated different components between Artemisiae Argyi Folium and Artemisiae Iwayomogii Herba. Results were used for classification of different species from collected samples.

Keyword

Artemisiae Argyi Folium; Artemisiae Iwayomogii Herba; HPLC-PDA; pattern analysis

MeSH Terms

Artemisia*
Classification
Discrimination (Psychology)*
Methods*

Figure

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  Fig. 1 Chemical structures of eight marker compounds 1 – 8 and an internal standard (I.S.).

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  Fig. 2 RP-HPLC chromatograms at UV 327 nm of marker compounds mixture (A), Artemisiae Argyi Folium (B; A10, and Artemisiae Iwayomogii Herba samples (C; I10).

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  Fig. 3 Pattern analysis of Artemisiae Iwayomogii Herba samples (n = 15) and Artemisiae Argyi Folium samples (n = 11).

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