Korean J Physiol Pharmacol.  2019 Jul;23(4):263-270. 10.4196/kjpp.2019.23.4.263.

Hepatoprotective effect of sodium hydrosulfide on hepatic encephalopathy in rats

Affiliations
  • 1Department of Pharmacology, College of Pharmacy, Chung-Ang University, Seoul 06974, Korea. udsohn@cau.ac.kr

Abstract

Hydrogen sulfide is well-known to exhibit anti-inflammatory and cytoprotective activities, and also has protective effects in the liver. This study aimed to examine the protective effect of hydrogen sulfide in rats with hepatic encephalopathy, which was induced by mild bile duct ligation. In this rat model, bile ducts were mildly ligated for 26 days. Rats were treated for the final 5 days with sodium hydrosulfide (NaHS). NaHS (25 µmol/kg), 0.5% sodium carboxymethyl cellulose, or silymarin (100 mg/kg) was administered intraperitoneally once per day for 5 consecutive days. Mild bile duct ligation caused hepatotoxicity and inflammation in rats. Intraperitoneal NaHS administration reduced levels of aspartate aminotransferase and alanine aminotransferase, which are indicators of liver disease, compared to levels in the control mild bile duct ligation group. Levels of ammonia, a major causative factor of hepatic encephalopathy, were also significantly decreased. Malondialdehyde, myeloperoxidase, catalase, and tumor necrosis factor-α levels were measured to confirm antioxidative and anti-inflammatory effects. N-Methyl-D-aspartic acid (NMDA) receptors with neurotoxic activity were assessed for subunit NMDA receptor subtype 2B. Based on these data, NaHS is suggested to exhibit hepatoprotective effects and guard against neurotoxicity through antioxidant and anti-inflammatory actions.

Keyword

Hepatic encephalopathy; Hydrogen sulfide; Inflammation

MeSH Terms

Alanine Transaminase
Ammonia
Animals
Aspartate Aminotransferases
Bile Ducts
Carboxymethylcellulose Sodium
Catalase
Hepatic Encephalopathy*
Hydrogen Sulfide
Inflammation
Ligation
Liver
Liver Diseases
Malondialdehyde
Models, Animal
N-Methylaspartate
Necrosis
Peroxidase
Rats*
Silymarin
Sodium*
Alanine Transaminase
Ammonia
Aspartate Aminotransferases
Carboxymethylcellulose Sodium
Catalase
Hydrogen Sulfide
Malondialdehyde
N-Methylaspartate
Peroxidase
Silymarin
Sodium
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