J Periodontal Implant Sci.  2019 Apr;49(2):114-126. 10.5051/jpis.2019.49.2.114.

Improvement of the osteogenic potential of ErhBMP-2-/EGCG-coated biphasic calcium phosphate bone substitute: in vitro and in vivo activity

• Hwang JH
• Oh S
• Kim S

• KMID: 2444223
• DOI: http://doi.org/10.5051/jpis.2019.49.2.114

Abstract

PURPOSE
The aim of this study was to evaluate the enhancement of osteogenic potential of biphasic calcium phosphate (BCP) bone substitute coated with Escherichia coli-derived recombinant human bone morphogenetic protein-2 (ErhBMP-2) and epigallocatechin-3-gallate (EGCG).
METHODS
The cell viability, differentiation, and mineralization of osteoblasts was tested with ErhBMP-2-/EGCG solution. Coated BCP surfaces were also investigated. Standardized, 6-mm diameter defects were created bilaterally on the maxillary sinus of 10 male New Zealand white rabbits. After removal of the bony windows and elevation of sinus membranes, ErhBMP-2-/EGCG-coated BCP was applied on one defect in the test group. BCP was applied on the other defect to form the control group. The animals were sacrificed at 4 or 8 weeks after surgery. Histologic and histometric analyses of the augmented graft and surrounding tissue were performed.
RESULTS
The 4-week and 8-week test groups showed more new bone (%) than the corresponding control groups (P<0.05). The 8-week test group showed more new bone (%) than the 4-week test group (P<0.05).
CONCLUSIONS
ErhBMP-2-/EGCG-coated BCP was effective as a bone graft material, showing enhanced osteogenic potential and minimal side effects in a rabbit sinus augmentation model.

Keyword

Biphasic calcium phosphate; Bone morphogenetic protein 2; Bone substitute; epigallocatechin-3-gallate

MeSH Terms

Animals
Bone Morphogenetic Protein 2
Bone Substitutes*
Calcium*
Cell Survival
Escherichia
Humans
In Vitro Techniques*
Male
Maxillary Sinus
Membranes
Miners
Osteoblasts
Rabbits
Transplants
Bone Morphogenetic Protein 2
Bone Substitutes
Calcium