Nat Prod Sci.  2019 Mar;25(1):59-63. 10.20307/nps.2019.25.1.59.

Hematopoiesis Activity of Sambucus javanica on Chloramphenicol-induced Aplastic Anemia Mouse Model

• Putra WE
• Rifa'i M

• KMID: 2443105
• DOI: http://doi.org/10.20307/nps.2019.25.1.59

Abstract

Hematopoiesis has a pivotal role in the maintenance of body homeostasis. Ironically, several hematological disorder caused by chemicals, drugs, and other environmental factors lead to severe bone marrow failure. Current treatments like stem cell transplantation and immunosuppression remain ineffective to ameliorate this diseases. Therefore, a newtreatment to overcome this entity is necessary, one of them by promoting the usage of medicinal plants. Thus, this study aimed to evaluate the hematopoiesis potency of S. javanica berries and leaves extracts in chloramphenicol (CMP)-induced aplastic anemia mice model. In this present study, several types of blood progenitor cell such as TER-119⁺VLA-4⁺ erythrocytes lineage, Gr-1⁺ granulocytes, and B220⁺ B-cell progenitor cells were evaluated by flow cytometry analysis. Accordingly, we revealed that S. javanica berries and leaves extracts significantly promoted TER-119⁺VLA-4⁺ erythrocytes lineage and Gr-1⁺ granulocytes after exposed by CMP. Thus, these results suggested that S. javanica berries and leaves extracts might have hematopoiesis activity in CMP-induced aplastic anemia mice model.

Keyword

Aplastic anemia; CMP; hematopoiesis; medicinal plant; S. javanica

MeSH Terms

Anemia, Aplastic*
Animals
B-Lymphocytes
Bone Marrow
Chloramphenicol
Erythrocytes
Flow Cytometry
Fruit
Granulocytes
Hematopoiesis*
Homeostasis
Immunosuppression
Iron
Mice*
Plants, Medicinal
Sambucus*
Stem Cell Transplantation
Stem Cells
Chloramphenicol
Iron

Figure

• 

  Fig. 1 A schematic diagram showed that CMP administration in BALB/c mouse induces acquired aplastic anemia, nevertheless the bioactive compounds from leaves and berries extracts of S. javanica can ameliorate the adverse condition by enhancing hematopoiesis.

• 

  Fig. 2 The representative flow cytometry diagrams show TER-119+VLA-4+, Gr-1+, and B220+ expression. S. javanica extracts promoted the relative number of TER-119+VLA-4+ erythrocytes lineage and Gr-1+ granulocytes, while CMP induce the relative number of B220+ B-cell progenitor cells. The bars are calculation of the relative number TER-119+VLA-4+, Gr-1+, and B220+ expression. The results were shown as the mean ± SD. *P<0.05, indicate significant difference. Treatment group in this study were vehicle, CMP 130 mg.kg−1 BW; BD1, CMP 130 mg.kg−1 BW+ berries extracts 50 mg.kg−1 BW; BD2, CMP 130 mg.kg−1 BW + berries extracts 100 mg.kg−1 BW; BD3, CMP 130 mg.kg−1 BW + berries extracts 200 mg.kg−1 BW; LD1, CMP 130 mg.kg−1 BW + leaves extracts 50 mg.kg−1 BW; LD2, CMP 130 mg.kg−1 BW + leaves extracts 100 mg.kg−1 BW; LD3, CMP 130 mg.kg−1 BW+ leaves extracts 200 mg.kg−1 BW.

Reference

1. Gupta V, Kumar A, Saini I, Saxena AK. Indian J Med Res. 2013; 137:502–506.

2. Young NS. Hematology Am Soc Hematol Educ Program. 2006; 72–77.

3. AlKhouri N, Ericson SG. Hosp Physician. 1999; 35:46–52.

4. Maluf E, Hamerschlak N, Cavalcanti AB, Júnior AA, Eluf-Neto J, Falcão RP, Lorand-Metze IG, Goldenberg D, Santana CL, Rodrigues Dde O, Passos LNM, Rosenfeld LGM, Pitta M, Loggetto S, Ribeiro AAF, Velloso ED, Kondo AT, Coelho EOM, Pintão MCT, de Souza HM, Borbolla JR, Pasquini R. Haematologica. 2009; 94:1220–1226.

5. Young NS, Kaufman DW. Haematologica. 2008; 93:489–492.

6. Muthukumaraswamy PK. Post Graduate Medicine. Bone marrow failure syndromes: An overview. India: Indian College of Physicians;2004. p. 372–382.

7. Rao KV. Pharmacotherapy: A Pathophysiologic Approach. 9E: Drug-induced hematologic disorders. USA: McGraw-Hill;2014. p. 359–374.

8. Turton JA, Andrews CM, Havard AC, Williams TC. Int J Exp Pathol. 2002; 83:225–238.

9. Gavrilu CI, Urian L, Petrov L. Acta Medica Transilvanica. 2015; 20:23–25.

10. Shukla P, Bansode FW, Singh RK. J Med Med Sci. 2011; 2:1313–1316.

11. Chen J. Clin Med Res. 2005; 3:102–108.

12. Yuan ZR, Shi Y. Cancer Res. 2008; 68:4875–4881.

13. Guinan EC. Hematology Am Soc Hematol Educ Program. 2011; 2011:76–81.

14. Segel GB, Lichtman MA. Williams Hematology. 8E: Aplastic anemia: Acquired and inherited. USA: McGraw-Hill;2010. p. 463–483.

15. Găman A, Găman G, Bold AT. Rom J Morphol Embryol. 2009; 50:669–674.

16. Young NS, Calado RT, Scheinberg P. Blood. 2006; 108:2509–2519.

17. Kamenjaković M, Hadžić V, Kulić S, Hasižić S. Food Health Disease. 2017; 6:1–6.

18. Ho GTT, Wangensteen H, Barsett H. Int J Mol Sci. 2017; 18:1–17.

19. Barak V, Halperin T, Kalickman I. Eur Cytokine Netw. 2001; 12:290–296.

20. Jiménez P, Tejero J, Cordoba-Diaz D, Quinto EJ, Garrosa M, Gayoso MJ, Girbés T. Toxins (Basel). 2015; 7:648–658.

21. Barsett H, Aslaksen TH, Gildhyal P, Michaelsen TE, Paulsen BS. European J Med Plants. 2012; 2:216–229.

22. Badescu M, Badulescu O, Badescu L, Ciocoiu M. Pharm Biol. 2015; 53:533–539.

23. Groza M, Ciocoiu M, Bădescu L, Bădulescu O, Bădescu M. Ann Rom Soc Cell Biol. 2010; 15:241–246.

24. Putra WE, Wafaretta E, Ardiana O, Januarisasi ID, Soewondo A, Rifa'i M. Biosci Res. 2017; 14:201–213.

25. Putra WE, Soewondo A, Rifa'i M. Jurnal Biotropika. 2015; 3:42–45.

26. Putra WE, Soewondo A, Rifa'i M. J Pure App Chem Res. 2015; 4:100–108.

27. Ogawa S. Blood. 2016; 128:337–347.

28. Jagannathan-Bogdan M, Zon LI. Development. 2013; 140:2463–2467.

29. Rieger MA, Schroeder T. Cold Spring Harb Perspect Biol. 2012; 4:1–18.

30. Velten L, Haas SF, Raffel S, Blaszkiewicz S, Islam S, Hennig BP, Hirche C, Lutz C, Buss EC, Nowak D, Boch T, Hofmann WK, Ho AD, Huber W, Trumpp A, Essers MA, Steinmetz LM. Nat Cell Biol. 2017; 19:271–281.

31. Orkin SH, Zon LI. Cell. 2008; 132:631–644.

32. Cheraghi M, Asadi-Samani M. Der Pharmacia Lettre. 2016; 8:393–399.

33. Udalamaththa VL, Jayasinghe CD, Udagama PV. Stem Cell Res Ther. 2016; 7:1–8.

34. Hasan NM, Al Sorkhy MK. IJHM. 2014; 1:18–21.

35. Pawar RS, Jain AP, Kashaw SK, Singhai AK. Indian J Pharm Sci. 2006; 68:337–340.

36. Mendi A, Yağci BG, Kiziloğlu M, Saraç N, Yilmaz D, Uğur A, Uçkan D. J Appl Oral Sci. 2017; 25:515–522.

37. Aksu DS, Didin M, Kayikci F. Revista Română de Medicin de Laborator. 2012; 20:47–57.

38. Ferri-Lagneau KF, Moshal KS, Grimes M, Zahora B, Lv L, Sang S, Leung T. PLoS One. 2012; 7:e39327.

39. Samet I, Villareal MO, Han J, Sayadi S, Isoda H. Asian J Biomed Pharm Sci. 2014; 4:1–7.