Biomol Ther.  2019 Feb;27(2):185-192. 10.4062/biomolther.2018.203.

Adipose-Derived Stem Cell Coculturing Stimulates Integrin-Mediated Extracellular Matrix Adhesion of Melanocytes by Upregulating Growth Factors

• Kim H
• Yi N
• Do BR
• Lee AY

• KMID: 2442010
• DOI: http://doi.org/10.4062/biomolther.2018.203

Abstract

Coculture with adipose-derived stem cells (ADSCs) can stimulate proliferation and migration of melanocytes. To enhance outcomes of skin disorders caused by melanocyte loss or death, mixed transplantation with ADSCs has been suggested. However, role of cocultured ADSCs in proliferation and migration of melanocytes remains unclear. This study determined the effect of ADSCs on production of growth factors and expression levels of intergrins in primary culture of adult human melanocytes with or without ADSCs and in nude mice grafted with such melanocytes. Higher amounts of growth factors for melanocytes, such as bFGF and SCF were produced and released from ADSCs by coculturing with melanocytes. Relative levels of integrins β1, α5, and α6 as well as adhesion to fibronectin and laminin were increased in melanocytes cocultured with ADSCs. Such increases were inhibited by neutralization of bFGF or SCF. Relative levels of bFGF, SCF and integrins were increased in nude mice skin after grafting with melanocyte+ADSC cocultures. Collectively, these results indicate that ADSCs can stimulate proliferation and migration of melanocytes by increasing expression of integrins in melanocytes through upregulation of production/release of melanocyte growth factors such as bFGF and SCF.

Keyword

Adipose-derived stem cell coculturing; Melanocytes; Growth factors; Integrins

MeSH Terms

Adult
Animals
Coculture Techniques
Extracellular Matrix*
Fibronectins
Humans
Integrins
Intercellular Signaling Peptides and Proteins*
Laminin
Melanocytes*
Mice
Mice, Nude
Skin
Stem Cells*
Transplants
Up-Regulation
Fibronectins
Integrins
Intercellular Signaling Peptides and Proteins
Laminin