Korean J Parasitol.  2019 Feb;57(1):33-38. 10.3347/kjp.2019.57.1.33.

Immune Response of BALB/c Mice toward Putative Calcium Transporter Recombinant Protein of Trichomonas vaginalis

• Mendoza-Oliveros T
• Arana-Argáez V
• Alvaréz-Sánchez LC
• Lara-Riegos J
• Alvaréz-Sánchez ME
• Torres-Romero JC

• KMID: 2441604
• DOI: http://doi.org/10.3347/kjp.2019.57.1.33

Abstract

Trichomoniasis is a common sexually transmitted infection caused by Trichomonas vaginalis, which actually does not exist a vaccine for control or prevention. Thus, the identification of new and potent immunogens in T. vaginalis, which can contribute to the development of a vaccine against this parasite, is necessary. Therefore, the aim of this work was to evaluate the potential of a recombinant Transient Receptor Potential-like channel of T. vaginalis (TvTRPV), as a promising immunogen in BALB/c mice. First, TvTRPV was cloned and expressed as a recombinant protein in Escherichia coli BL21 cells and purified by nickel affinity. Next, BALB/c mice were immunized and the antibody levels in mice serum and cytokines from the supernatant of macrophages and from co-culture systems were evaluated. Recombinant TvTRPV triggered high levels of specific total IgG in sera from the immunized mice. Also, a statistically significant increase of cytokines: IL-1β, IL-6, and TNF-α after stimulation with the corresponding antigens in vitro, was identified. Moreover, co-cultures using CD4⁺ T cells from immunized mice were able to identify higher levels of IL-10 and IFN-γ. These results were useful to validate the immunogenicity of TvTRPV in BALB/c mice, where IL-10-IFN-γ-secreting cells could play a role in infection control, supporting the potential of TvTRPV as a promising target for vaccine against T. vaginalis.

Keyword

Trichomonas vaginalis; TRPV channel; recombinant protein; immune response; BALB/c mouse

MeSH Terms

Animals
Calcium*
Clone Cells
Coculture Techniques
Cytokines
Escherichia coli
Immunoglobulin G
In Vitro Techniques
Infection Control
Interleukin-10
Interleukin-6
Macrophages
Mice*
Nickel
Parasites
Sexually Transmitted Diseases
T-Lymphocytes
Trichomonas vaginalis*
Trichomonas*
Calcium
Cytokines
Immunoglobulin G
Interleukin-10
Interleukin-6
Nickel