Korean J Parasitol.
1992 Dec;30(4):323-328. 10.3347/kjp.1992.30.4.323.
Comparative antigen analysis of Trichomonas vaginalis by enzyme-linked immunoelectrotransfer blot technique
- Affiliations
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- 1Department of Parasitology, College of Medicine, Hanyang University, Seoul, Korea.
- KMID: 1715511
- DOI: http://doi.org/10.3347/kjp.1992.30.4.323
Abstract
- Analysis of six isolates of Trichomonas vaginalis was carried out with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and enzyme-linked immunoelectrotransfer blot (EITB). Trichloroacetic acid-treated antigens of the 6 isolates revealed 25 protein profiles ranging 12-170 kDa of molecular weight in SDS-PAGE. In EITB, the specific immunogenic bands were visualized at 51 kDa and 96 kDa when HY-1 antigen was probed with different mice sera immunized with 6 isolates of T. vaginalis. The banding patterns with different sera showed isolate-to-isolate variability. In EITB, homologous antigen (HY-1) did not show any enhanced response in reacting to homologous antiserum (HY-1) when 6 isolates of T. vaginalis were probed with a single serum (HY-1). It is assumed that the different banding patterns of six isolates show isolate-to-isolate variability and immunogenic common bands in 41, 47, 74 and 94 kDa on EITB may connote the important significance on immune response in T. vaginalis infection.
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