Korean J Parasitol.  2017 Aug;55(4):375-384. 10.3347/kjp.2017.55.4.375.

Trichomonas vaginalis α-Actinin 2 Modulates Host Immune Responses by Inducing Tolerogenic Dendritic Cells via IL-10 Production from Regulatory T Cells

• Lee HY
• Kim J
• Ryu JS
• Park SJ

• KMID: 2389972
• DOI: http://doi.org/10.3347/kjp.2017.55.4.375

Abstract

Trichomonas vaginalis is a pathogen that triggers severe immune responses in hosts. T. vaginalis α-actinin 2, Tvα-actinin 2, has been used to diagnose trichomoniasis. This study was undertaken to examine the role of Tvα-actinin 2 as an antigenic molecule to induce immune responses from humans. Western blot analysis using anti-Tvα-actinin 2 antibodies indicated its presence in the secreted proteins of T. vaginalis. ELISA was employed to measure cytokine production by vaginal epithelial cells, prostate cells, mouse dendritic cells (DCs), or T cells stimulated with T. vaginalis or Tvα-actinin 2 protein. Both T. vaginalis and rTvα-actinin 2 induced cytokine production from epithelial cell lines, including IL-10. Moreover, CD4+CD25− regulatory T cells (Treg cells) incubated with rTvα-actinin 2-treated DCs produced high levels of IL-10. These data indicate that Tvα-actinin 2 modulates immune responses via IL-10 production by Treg cells.

Keyword

Trichomonas vaginalis; α-actinin 2; Treg cell; interleukin-10 (IL-10)

MeSH Terms

Animals
Antibodies
Blotting, Western
Dendritic Cells*
Enzyme-Linked Immunosorbent Assay
Epithelial Cells
Humans
Interleukin-10*
Mice
Prostate
T-Lymphocytes
T-Lymphocytes, Regulatory*
Trichomonas vaginalis*
Trichomonas*
Antibodies
Interleukin-10