Korean J Parasitol.  2016 Feb;54(1):71-74. 10.3347/kjp.2016.54.1.71.

Influence of 120 kDa Pyruvate:Ferredoxin Oxidoreductase on Pathogenicity of Trichomonas vaginalis

• Song HO

• KMID: 2374020
• DOI: http://doi.org/10.3347/kjp.2016.54.1.71

Abstract

Trichomonas vaginalis is a flagellate protozoan parasite and commonly infected the lower genital tract in women and men. Iron is a known nutrient for growth of various pathogens, and also reported to be involved in establishment of trichomoniasis. However, the exact mechanism was not clarified. In this study, the author investigated whether the 120 kDa protein of T. vaginalis may be involved in pathogenicity of trichomonads. Antibodies against 120 kDa protein of T. vaginalis, which was identified as pyruvate:ferredoxin oxidoreductase (PFOR) by peptide analysis of MALDI-TOF-MS, were prepared in rabbits. Pretreatment of T. vaginalis with anti-120 kDa Ab decreased the proliferation and adherence to vaginal epithelial cells (MS74) of T. vaginalis. Subcutaneous tissue abscess in anti-120 kDa Ab-treated T. vaginalis-injected mice was smaller in size than that of untreated T. vaginalis-infected mice. Collectively, the 120 kDa protein expressed by iron may be involved in proliferation, adhesion to host cells, and abscess formation, thereby may influence on the pathogenicity of T. vaginalis.

Keyword

Trichomonas vaginalis; pyruvate:ferredoxin oxidoreductase; iron; adhesion; abscess formation

MeSH Terms

Animals
Antibodies/metabolism
Cell Proliferation/drug effects
Epithelial Cells/parasitology
Host-Pathogen Interactions/drug effects/*physiology
Iron/pharmacology
Mice
Pyruvate Synthase/*metabolism
Rabbits
Trace Elements/pharmacology
Trichomonas Infections/*parasitology
Trichomonas vaginalis/drug effects/genetics/metabolism/*pathogenicity
Antibodies
Iron
Pyruvate Synthase
Trace Elements