Korean J Parasitol.  2006 Dec;44(4):373-378. 10.3347/kjp.2006.44.4.373.

Hydrogenosomal activity of Trichomonas vaginalis cultivated under different iron conditions

• Kim YS
• Song HO
• Choi IH
• Park SJ
• Ryu JS

• KMID: 1088770
• DOI: http://doi.org/10.3347/kjp.2006.44.4.373

Abstract

To evaluate whether iron concentration in TYM medium influence on hydrogenosomal enzyme gene expression and hydrogenosomal membrane potential of Trichomonas vaginalis, trophozoites were cultivated in irondepleted, normal and iron-supplemented TYM media. The mRNA of hydrogenosomal enzymes, such as pyruvate ferredoxin oxidoreductase (PFOR), hydrogenase, ferredoxin and malic enzyme, was increased with iron concentrations in T. vaginalis culture media, measured by RT-PCR. Hydrogenosomal membrane potentials measured with DiOC6 also showed similar tendency, e.g. T. vaginalis cultivated in iron-depleted and iron-supplemented media for 3 days showed a significantly reduced and enhanced hydrogenosomal membrane potential compared with that of normal TYM media, respectively. Therefore, it is suggested that iron may regulate hydrogenosomal activity through hydrogenosomal enzyme expression and hydrogenosomal membrane potential.

Keyword

Trichomonas vaginalis; hydrogenosomal enzyme; gene expression; hydrogenosomal membrane potential; iron; DiOC6; PFOR; hydrogenase; malic enzyme; ferredoxin

MeSH Terms

Trichomonas vaginalis/*growth & development
Reverse Transcriptase Polymerase Chain Reaction
Pyruvate Synthase/genetics/metabolism
Organelles/*enzymology/metabolism/*physiology
Membrane Potentials
Malate Dehydrogenase/genetics/metabolism
Iron/*metabolism
Hydrogenase/genetics/metabolism
Hydrogen/*metabolism
Humans
Gene Expression Regulation, Enzymologic
*Gene Expression Regulation
Ferredoxins/genetics/metabolism
Culture Media
Animals