J Korean Soc Plast Reconstr Surg.  2010 Jan;37(1):7-11.

Vascular Endothelial Growth Factor Effect on Notch 1 Expression and Proliferation of Fibroblast

• Koh SH

Abstract

PURPOSE: Vascular endothelial growth factor (VEGF) is known as a growth factor of endothelium and fibroblast. The purpose is to know the VEGF effects on fibroblast proliferation and fibroblast's notch receptor expression.
METHODS
CCD-986sk fibroblast was purchased from the Korean Cell Bank and was used in XTT assay for proliferation and wound healing assay for migration. Immunofluorescent (IF) staining and western blotting were used in testing notch expression of fibroblast. Semiquantitative RT-PCR was used in checking notch 1 mRNA production by fibroblast. Student-t test was used for analyzing results.
RESULTS
Cell proliferation assay using XTT showed significant higher proliferation in VEGF treated fibroblast, 2.324 +/- 0.0026 vs. 2.463 +/- 0.017 (p=0.002). Wound healing assay showed longer migration in VEGF treated fibroblast (p=0.062). The fluorescence was brighter in VEGF treated cells of notch 1 IF staining. Notch 1 expressions and mRNA productions increased more in VEGF treated cells.
CONCLUSION
VEGF stimulates fibroblast to proliferate, migrate and to express Notch 1 simultaneously. Notch receptor could be related to VEGF mediated wound healing.

Keyword

Fibroblast; Notch receptor; Vascular endothelial growth factor

MeSH Terms

Blotting, Western
Cell Proliferation
Endothelium
Fibroblasts
Fluorescence
RNA, Messenger
Vascular Endothelial Growth Factor A
Wound Healing
RNA, Messenger
Vascular Endothelial Growth Factor A