Korean J Otorhinolaryngol-Head Neck Surg.  2018 Aug;61(8):412-420. 10.3342/kjorl-hns.2017.01067.

Role of Interleukin-10-Expressing B Cells on the Pathophysiology of Chronic Sinusitis

• Park HS
• Park SK
• Yeon SH
• Xu J
• Rha KS
• Kim YM

• KMID: 2436518
• DOI: http://doi.org/10.3342/kjorl-hns.2017.01067

Abstract

BACKGROUND AND OBJECTIVES
Many kinds of inflammatory cells and cytokines are suggested to be related with pathophysiology of chronic rhinosinusitis with nasal polyp (CRSwNP), but its mechanism is not yet fully understood. In particular, little is known about the function and role of Interleukin-10 (IL-10) producing regulatory B cells. The aim of this study was to investigate the presence and function of B cells, especially IL-10-producing regulatory B cells, in the nasal polyp (NP) and nasal mucosa.
SUBJECTS AND METHOD
Twenty patients with CRSwNP, 10 patients with chronic rhinosinusitis without nasal polyp (CRSsNP) and 10 control subjects were enrolled in this study. NP tissues and uncinate tissues were collected for analysis. Real-time polymerase chain reaction (RT-PCR), immunohistochemistry were performed to measure the expression levels of the selected inflammatory cytokines and inflammation-associated molecules.
RESULTS
In the mucosal tissues of CRSsNP patients, the number of IL-10+ B cells was significantly lower than that of NP and control mucosa of CRSwNP patients. The number of IL-10+ B cells was significantly increased in the eosinophilic NP, non-eosinohilic NP, CRSsNP, and control groups. There was a significant positive correlation between the number of IL-10+ B cells and B cell activating factor (BAFF).
CONCLUSION
The expression of IL-10+ B cells and BAFF was significantly increased in CRSwNP patients compared to CRSsNP patients and control mucosal tissues, and IL-10+ B cells were more significantly increased in eosinophilic tissues. These results indicate that regulatory B cells are involved in the pathophysiology of eosinophilic NP and that BAFF contributes to the production of regulatory B cells.

Keyword

B-lymphocytes; Interleukin-10; Rhinitis; Sinusitis

MeSH Terms

B-Cell Activating Factor
B-Lymphocytes*
B-Lymphocytes, Regulatory
Cytokines
Eosinophils
Humans
Immunohistochemistry
Interleukin-10
Methods
Mucous Membrane
Nasal Mucosa
Nasal Polyps
Real-Time Polymerase Chain Reaction
Rhinitis
Sinusitis*
B-Cell Activating Factor
Cytokines
Interleukin-10