J Korean Ophthalmol Soc.  2010 May;51(5):746-750.

Effect of High Glucose on the Production of Reactive Oxygen Species in R28 Cells

• Lee JM
• Kim JW

Abstract

PURPOSE
To investigate the effect of high glucose (HG) on the production of reactive oxygen species (ROS) in retinal precursor R28 cells.
METHODS
R28 cells were incubated with low glucose (5 mM) or HG (25 mM) for two days. Additionally, the cells were co-exposed to 50 micrometer N-acetyl cysteine or 100 micrometer L-arginine. Production of nitric oxide (NO), ROS, and superoxide were assessed by Griess assay, DCFH-DA assay, and modified cytochrome c assay, respectively.
RESULTS
HG increased the production of NO, ROS, and superoxide, which were abolished by antioxidants NAC and L-arginine (a substrate for NO production).
CONCLUSIONS
HG increased ROS production in R28 cells. Thus, HG may cause cellular dysfunction and damage by inducing oxidative stress in retinal ganglion cells.

Keyword

High glucose; Oxidative stress; Reactive oxygen species; Retinal ganglion cells

MeSH Terms

Antioxidants
Arginine
Cysteine
Cytochromes c
Fluoresceins
Glucose
Nitric Oxide
Oxidative Stress
Reactive Oxygen Species
Retinal Ganglion Cells
Retinaldehyde
Superoxides
Antioxidants
Arginine
Cysteine
Cytochromes c
Fluoresceins
Glucose
Nitric Oxide
Reactive Oxygen Species
Retinaldehyde
Superoxides

Figure

• 

  Figure 1. Effect of glucose on the production of nitric oxide in R28 cells. High glucose (25 mM) induced significantly more production of nitric oxide than low glucose (5 mM). (*p<0.05)

• 

  Figure 2. High glucose (HG, 25 mM) induced more production of superoxide than low glucose (LG, 5 mM) (* p>0.05). HG-induced increased production of superoxide was abolished significantly by co-exposed N-acetyl cysteine (NAC) or L-arginine (Arg), respectively. (** p<0.05)

• 

  Figure 3. High glucose (HG, 25 mM) induced more production of reactive oxygen species (ROS) than low glucose (LG, 5 mM) (* p>0.05). HG-induced increased production of ROS was abolished significantly by co-exposed N-acetyl cysteine (NAC) or L-arginine (Arg), respectively. (** p<0.05)

Reference

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