Tissue Eng Regen Med.
2018 Oct;15(5):615-627. 10.1007/s13770-018-0153-7.
The Effect of Fibronectin-Immobilized Microgrooved Titanium Substrata on Cell Proliferation and Expression of Genes and Proteins in Human Gingival Fibroblasts
- Affiliations
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- 1Department of Oral and Maxillofacial Pathology and Institute of Oral Biology, School of Dentistry, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, Republic of Korea.
- 2Doctorplant Dental Clinic, 885 Gyeongin-ro, Yeongdeungpogu, Seoul 07303, Republic of Korea.
- 3Energy and Environmental Division, Korea Institute of Ceramic Engineering and Technology, 101 Soho-ro, Jinju-si, Gyeongsangnam-do 52851, Republic of Korea.
- 4Department of Maxillofacial Biomedical Engineering and Institute of Oral Biology, School of Dentistry, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, Republic of Korea. schlee@khu.ac.kr
- 5Core Research Laboratory, Clinical Research Institute, Kyung Hee University Hospital at Gangdong, 892 Dongnamro, Gangdong-gu, Seoul 05278, Republic of Korea.
- 6Department of Biomaterials and Prosthodontics, Kyung Hee University Hospital at Gangdong, Institute of Oral Biology, School of Dentistry, Kyung Hee University, 892 Dongnamro, Gangdong-gu, Seoul 05278, Republic of Korea. ysprosth@hanmail.net
- 7Department of Prosthodontics, Dankook University Sejong Dental Hospital, 87 Doum 8-ro, Sejong 30107, Republic of Korea.
- 8Department of Dentistry, National Health Insurance Medical Center Ilsan Hospital, 100 Ilsan-ro, Ilsandong-gu, Goyang-si, Gyeonggi-do 10444, Republic of Korea.
- 9Department of Oral and Maxillofacial Surgery, Kyung Hee University Hospital at Gangdong, Institute of Oral Biology, School of Dentistry, Kyung Hee University, 892 Dongnamro, Gangdong-gu, Seoul 05278, Republic of Korea.
- KMID: 2422675
- DOI: http://doi.org/10.1007/s13770-018-0153-7
Abstract
- BACKGROUND
We aimed to determine the effect of fibronectin (FN)-immobilized microgrooved titanium (Ti) on human gingival fibroblast proliferation, gene expression and protein expression.
METHODS
Photolithography was used to fabricate the microgrooved Ti, and amine funtionalization (silanization) was used for FN immobilization on titanium surfaces. Cell proliferation, gene expression and protein expression were analyzed, followed by multiple regression analysis for determining the influential factors on cell proliferation.
RESULTS
FN-immobilized microgrooved Ti significantly enhanced the fibroblast proliferation in various timelines of culture, among which a burst of fivefold increase is induced at 96 h of culture compared to that on the control smooth Ti. We suggest a presence of the synergistic promotion effect of microgrooves and FN immobilization on fibroblast proliferation. Through a series of analyses on the expression of various genes and proteins involved in cell adhesion and proliferation, cyclin-dependent kinase 6, cyclin D1, integrin α5, oncogene c-Src, osteonectin, paxillin and talin-2 were determined as influential factors on promoting fibroblast proliferation induced by FN-immobilized microgrooved Ti.
CONCLUSION
FN-immobilized microgrooved Ti can act as an effective surface for enhancing fibroblast proliferation, and can be used for promoting soft tissue response on the connective tissue attachment zone of biomaterial surfaces.
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