Tissue Eng Regen Med.  2018 Oct;15(5):591-599. 10.1007/s13770-018-0137-7.

Cellular Response of Anodized Titanium Surface by Poly(Lactide-co-Glycolide)/Bone Morphogenic Protein-2

• Lee SY
• Koak JY
• Kim SK
• Heo SJ

• KMID: 2422673
• DOI: http://doi.org/10.1007/s13770-018-0137-7

Abstract

BACKGROUND
The purpose of this study is to examine physical characteristics of and initial biological properties to anodized titanium treated with poly(D,L-lactide-co-glycolide) (PLG) mixed with recombinant human bone morphogenic protein-2 (rhBMP-2).
METHODS
Titanium specimens were prepared in groups of four as follows: group NC was anodized under 300 V as control; group PC was anodized then dropped and dried with solution 0.02 ml PLG; group D was anodized then dropped and dried with solution 0.02 ml PLG/rhBMP-2 (3.75 µg per disc); and group E was anodized then coated with 0.02 ml PLG/rhBMP-2 (3.75 µg per disc) by electrospray. Human osteoblastic-like sarcoma cells were cultured. Cell proliferation and alkaline phosphatase (ALP) activity test were carried out. Runx-2 gene was investigated by the reverse transcription-polymerase chain reaction. Immunofluorescence outcome of osteogenic proteins was observed.
RESULTS
After 3 days, there were significantly higher proliferations compared rhBMP-2 loaded titanium discs with rhBMP-2 unloaded discs. The ALPase activity on rhBMP-2 loaded titanium discs was significantly higher than in rhBMP-2 unloaded discs. The expression level of Runx2 mRNA presented the highest on the PLG/rhBMP-2-coated surface.
CONCLUSION
PLG polymers mixed with rhBMP-2 might improve proliferation, differentiation and osteogenic protein formation of cells on the anodized titanium.

Keyword

Bone morphogenic protein; Polymer; Anodized titanium; Cell proliferation; Cell differentiation

MeSH Terms

Alkaline Phosphatase
Cell Differentiation
Cell Proliferation
Fluorescent Antibody Technique
Humans
Polymers
RNA, Messenger
Sarcoma
Titanium*
Alkaline Phosphatase
Polymers
RNA, Messenger
Titanium