J Korean Acad Prosthodont.  2016 Apr;54(2):120-125. 10.4047/jkap.2016.54.2.120.

An in vitro study of mesenchymal stem cell proliferation on titanium discs coated with rhTGF-β2/PLGA by electrospray

• Kim J
• Kim SK
• Heo SJ
• Koak JY
• Lee WS
• Lee JH
• Park JM

• KMID: 2162387
• DOI: http://doi.org/10.4047/jkap.2016.54.2.120

Abstract

PURPOSE
The purpose of this study is to identify the effect of mesenchymal stem cell proliferation on recombinant human transforming growth factor-beta (rhTGF-β2) / poly (D,L-lactide-co-glycolide) (PLGA) treated titanium discs by electrospray.
MATERIALS AND METHODS
Anodized titanium surface coated with PLGA was used for a control group to compare anodized titanium surface coated with 125 ng/ml and 500 ng/ml rhTGF-β2 as test groups. Atomic force microscope (AFM) test was utilized to determine the difference in coating surface roughness, and field-emission scanning electron microscopy (FE-SEM) was taken to visualize even distribution of coating particles on titanium discs. The mesenchymal stem cell proliferation was tested by using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl-tetrazolium bromide) assay on 1st, 4th, 7th days.
RESULTS
According to AFM results, there was no statistically significant difference in titanium discs treated with PLGA and with rhTGF-β2/PLGA (P>.05). MTT assay test results showed that there was statistically significant difference in mesenchymal stem cell proliferation on test groups compared to control groups at 7th day, and cell viability on discs coated with rhTGF-β2 was significantly higher than control groups (P<.05).
CONCLUSION
Titanium surface coated with rhTGF-β2/PLGA shows statistically significant higher cell proliferation and the titanium surface coated with the higher concentration of rhTGF-β2 presents faster cell growth activity.

Keyword

Titanium; rhTGF-β2; PLGA; Mesenchymal stem cells

MeSH Terms

Cell Proliferation
Cell Survival
Humans
Mesenchymal Stromal Cells*
Microscopy, Electron, Scanning
Titanium*
Titanium

Figure

• 

  Fig. 1. SEM image of anodized titanium implant coated by electrospray (×5,000). (A) Anodized Ti disc, (B) Anodized Ti disc coated with 40 ㎕rhTGF-β 2 (500 ng/ml) submicron particles by electrospray technique.

• 

  Fig. 2. The results of surface roughness measured by AFM (10 ㎛× 10 ㎛). (A) Anodized titanium disc, (B) Anodized titanium disc coated with 40 ㎕rhTGF-β 2 (500 ng/ml per disc).

• 

  Fig. 3. MTT assay results. The result presents that there was tendency of cell proliferation increasing by time, however; there was no statistically significant difference among groups in 1 day and 4 days. At the 7 days of cell culture, there was statistically significant difference between control groups and experimental groups (∗P<.05, ∗∗P<.05)

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