J Korean Acad Prosthodont.  2016 Apr;54(2):120-125. 10.4047/jkap.2016.54.2.120.

An in vitro study of mesenchymal stem cell proliferation on titanium discs coated with rhTGF-β2/PLGA by electrospray

Affiliations
  • 1Department of Prosthodontics, Seoul National University Dental Hospital, School of Dentistry, Seoul National University, Seoul, Republic of Korea. ksy0617@snu.ac.kr
  • 2Department of Medical Science, Major in Dentistry, Ulsan University, Seoul, Republic of Korea.
  • 3Department of Prosthodontics and Dental Research Institute, Seoul National University Gwanak Dental Hospital, Seoul, Republic of Korea.

Abstract

PURPOSE
The purpose of this study is to identify the effect of mesenchymal stem cell proliferation on recombinant human transforming growth factor-beta (rhTGF-β2) / poly (D,L-lactide-co-glycolide) (PLGA) treated titanium discs by electrospray.
MATERIALS AND METHODS
Anodized titanium surface coated with PLGA was used for a control group to compare anodized titanium surface coated with 125 ng/ml and 500 ng/ml rhTGF-β2 as test groups. Atomic force microscope (AFM) test was utilized to determine the difference in coating surface roughness, and field-emission scanning electron microscopy (FE-SEM) was taken to visualize even distribution of coating particles on titanium discs. The mesenchymal stem cell proliferation was tested by using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl-tetrazolium bromide) assay on 1st, 4th, 7th days.
RESULTS
According to AFM results, there was no statistically significant difference in titanium discs treated with PLGA and with rhTGF-β2/PLGA (P>.05). MTT assay test results showed that there was statistically significant difference in mesenchymal stem cell proliferation on test groups compared to control groups at 7th day, and cell viability on discs coated with rhTGF-β2 was significantly higher than control groups (P<.05).
CONCLUSION
Titanium surface coated with rhTGF-β2/PLGA shows statistically significant higher cell proliferation and the titanium surface coated with the higher concentration of rhTGF-β2 presents faster cell growth activity.

Keyword

Titanium; rhTGF-β2; PLGA; Mesenchymal stem cells

MeSH Terms

Cell Proliferation
Cell Survival
Humans
Mesenchymal Stromal Cells*
Microscopy, Electron, Scanning
Titanium*
Titanium
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